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MABF505 Anti-CAMK4, clone 8C5B8 Antibody

MABF505
100 µL  
Purchase on Sigma-Aldrich

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Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IF, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF505
DescriptionAnti-CAMK4, clone 8C5B8 Antibody
Alternate Names
  • Calcium/calmodulin-dependent protein kinase type IV
  • CaMK IV
  • CAMK4
Background InformationCalcium/calmodulin-dependent protein kinase type IV (CaMK IV), also known as CaM kinase-GR, and encoded by the gene name CAMK4 or CAMK or CAMK-GR or CAMKIV, is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. CAMK4 is localized in hippocampal neuron nuclei and is associated with chromatin and the nuclear matrix in spermatids. Expression in CAMK4 can be found in brain, thymus, CD4 T-cells, testis, and epithelial ovarian cancer tissue. CAMK4 can be phosphorylated by CaMKK1 and CaMKK2 on Thr200 and dephosphorylated by protein phosphatase 2A.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in PBS containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CAMK4, clone 8C5B8 Antibody is validated for use in western blotting, IHC, immunofluorescence & ELISA for the detection of CAMK4.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in ovarian and rectum cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human CAMK4 expressed in E. Coli
Clone8C5B8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CAMK4
  • CAMK
  • CAMK-GR
  • CAMKIV
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SK-N-SH, Raji and HeLa cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CAMK4 in SK-N-SH, Raji and HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABF505 04053252957154