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MABE1529 Anti-BRD7 Antibody, clone BRM 2D3

This Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7.

Anti-BRD7 Antibody, clone BRM 2D3 : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: BRD7, BP75, CELTIX1 UniProt Number: Q9NPI1
MABE1529
100 μg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1529
ReplacesMABE66
DescriptionAnti-BRD7 Antibody, clone BRM 2D3
Alternate Names
  • BRD7
  • Bromodomain-containing protein 7
  • 75 kDa bromodomain protein
  • Protein CELTIX-1
Background InformationBromodomain-containing protein 7 (UniProt Q9NPI1; also known as 75 kDa bromodomain protein, Protein CELTIX-1) is encoded by the BRD7 (also known as BP75, CELTIX1) gene (Gene ID 29117) in human. BRD7 is a ubiquitously expressed single bromodomain-/BRD-containing member of the family of BRD-containing proteins. BRD7 functions as a subunit of the SWI/SNF chromatin-remodeling complex involved in transcription regulation. It also interacts with the well known tumor suppressor p53 to trans-activate genes involved in cell cycle arrest. BRD7 is downregulated in colorectal carcinoma and nasopharyngeal carcinoma, and BRD7 overexpression is reported to inhibit nasopharyngeal carcinoma cell proliferation via cell cycle arrest by transcriptionally regulating ras/MEK/ERK, Rb/E2F, b-catenin and ERK pathways. BRD7 mRNA has been identified as a silencing target of miR-300, overexpression of miR-300 promotes the proliferation, invasion and EMT of osteosarcoma cells through targeting BRD7 mRNA.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected BRD7 in 10 µg of HEK293 and HeLa cell lysates.
Immunoprecipitation Analysis: A representative lot immunoprecipitated BRD7 from H1299 cell lysate (Courtesy of Thomas Dobner, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, D-93053 Regensburg, Germany).
Biological Information
ImmunogenRecombinant human BRD7.
CloneBRM203
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone BRM 2D3 detects human 75 kDa bromodomain protein, also known as protein CELTIX-1.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BRD7
  • BP75
  • CELTIX1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~75 kDa observed. 74.14 kDa (isoform 1), 74.27 kDa (isoform 2) calculated. Added Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected BRD7 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABE1529 04055977355451