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MABN1838 Anti-AGE Antibody, Methylglyoxal Antibody, clone 3D11

Anti-AGE, Methylglyoxal, clone 3D11, Cat. No. MABN1838, is a highly specific mouse monoclonal antibody that targets Methylglyoxal modified proteins and has been tested in ELISA and Immunohistochemistry (Paraffin).

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Research Category: Neuroscience
MABN1838
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIH(P), ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1838
DescriptionAnti-AGE Antibody, Methylglyoxal Antibody, clone 3D11
Alternate Names
  • Methylglyoxal
  • AGE Product methylglyoxal
  • clone 3D11
Background InformationChronic accumulation of Advanced Glycation End products (AGEs) is a common feature during aging, hyperlipidemia, and diabetes and affects function of proteins, lipids, and DNA. Although formation of AGE products is considered to be predominantly an endogenous process, certain external factors, such as some dietary products and smoking can increase the level of AGE in tissues. Under certain conditions glucose may form covalent adducts with the plasma proteins through the process of glycation. Glycation is a non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of proteins. The term AGE often refers to non-reactive terminal products, such as N- carboxymethyllysine (CML) as well as reactive precursors, such as methylglyoxal (MG), which is generated through a non-oxidative process from triose phosphates during anaerobic glycolysis. MG reacts with arginine, lysine, and cysteine residues in proteins to form AGE products. Excessive glycation can disrupt protein function by altering enzyme activity, receptor function, and molecular conformation. Protein glycation and subsequent formation of AGE products contributes to the pathogenesis of diabetes-related complications, such as retinopathy, nephropathy, and neuropathy. AGE products interact with a variety of cell-surface AGE-binding receptors (RAGE) that results in their endocytosis and degradation. It can also generate pro-oxidant and pro-inflammatory events in cells. AGE products could also contribute to the development or worsening of many degenerative diseases, including Alzheimer's disease. (Ref.: Cai,W., et al. (2002). Mol. Med 8 (7): 337-346).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-AGE, Methylglyoxal, clone 3D11, Cat. No. MABN1838, is a highly specific mouse monoclonal antibody that targets Methylglyoxal modified proteins and has been tested in ELISA and Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesELISA Analysis: A representative lot detected AGE, Methylglyoxal in ELISA applications (Vlassara, H., et. al. (2002). Proc Natl Acad Sci USA. 99(24):15596-601; Cai, W., et. al. (2008). Am J Pathol. 173(2):327-36; Cai, W., et. al. (2012). Proc Natl Acad Sci USA. 109(39):15888-15893).
Biological Information
ImmunogenMethylglyoxal-cojugated Ovalbumin.
Clone3D11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3D11 specifically reacts with methylglyoxal modified proteins. It does not react with CML, CEL, or other AGE epitopes.
IsotypeIgG2aλ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human and Rat.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse kidney tissue.

Immunohistochemistry Analysis: A 1:3,000 dilution of this antibody detected AGE, Methylglyoxal in kidney tissue from mice fed high AGE diets.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABN1838 04054839178924