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UFC910024 Amicon Ultra-15, membrana PLHK Ultracel-PL, 100 kDa

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Aplicaciones clave: Extracción de proteínas, Purificación de proteínas
UFC910024
24  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Key ApplicationsLímite de peso molecular nominal (NMWL)VolumenVolumen de concentrado final mínimo
Extracción de proteínas, Purificación de proteínas100 KDa15 mL200 µL
Descripción
Número de catálogoUFC910024
Nombre comercial
  • Amicon Ultra
DescripciónAmicon Ultra-15, membrana PLHK Ultracel-PL, 100 kDa
Información preliminarAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC910008 - 8 pack
UFC910024 - 24 pack
UFC910096 - 96 pack



High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
Referencias bibliográficas
Información del producto
Código del filtroPLHK
Código de coloresTransparente con tapa rosa
Tipo de filtroUltrafiltración
Límite de peso molecular nominal (NMWL)100 KDa
Quality LevelMQ400
Aplicaciones
Aplicación Ultracel-100 regenerated cellulose membrane, 15 mL sample volume
Aplicaciones clave
  • Extracción de proteínas
  • Purificación de proteínas
Notas de aplicaciónRotor de cabezal basculante: 4.000 x g; Rotor de ángulo fijo (35°): 5.000 g
Información biológica
EsterilidadNo estéril
Información fisicoquímica
Aplicación centrífugaProtein Concentration and Desalting
Dimensiones
Longitud12.1 cm (4.807 in.)
Diámetro2.97 cm
Área de filtración7.6 cm²
Volumen de concentrado final mínimo200 µL
Volumen15 mL
Información de materiales
Química
  • Regenerated Cellulose (RC)
Material del dispositivo
  • Housing: Styrene/butadiene
  • Tubo: Polipropileno
  • Tapa: Polietileno
Material de soporteCelulosa regenerada
Información toxicológica
Información de seguridad según el GHS
Información de seguridad
Declaraciones de uso del producto
Información de almacenamiento y transporte
Información sobre embalaje
Cantitad24
Información de transporte
Información complementaria
Especificaciones
Global Trade Item Number
Número de referencia GTIN
UFC910024 04053252317439

Documentation

Referencias bibliográficas

Visión general referenciasPub Med ID
Specific Lipids Modulate the Transporter Associated with Antigen Processing (TAP).
Schölz, Christian, et al.
J. Biol. Chem., 286: 13346-56 (2011)  2010

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Certificados de calidad

Cargo
UFC910024 - 0000241948

Folleto

Cargo
REFINE PROTEIN PREPARATION. Tools for better protein analysis

Preguntas frecuentes

PreguntaRespuesta
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
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Manuales del usuario

Cargo
Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide