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14-502M Akt3/PKBγ (S472D) Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant human Akt3/PKBγ, amino acids 117-end, containing a S472D mutation. For use in Kinase Assays.

14-502M
250 µg  Also available in 10 μg size (cat#14-502) and in bulk (cat# 14-502-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-502M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAkt3/PKBγ (S472D) Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant human Akt3/PKBγ, amino acids 117-end, containing a S472D mutation.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant human Akt3/PKBγ, amino acids 117-end, containing a S472D mutation. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman Akt3/PKBγ expressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Gene Symbol
  • AKT3
  • PRKBG
  • DKFZP434N0250
  • PKBG
  • RAC-gamma
  • RAC-PK-gamma
  • STK-2
Protein TargetAkt3/PKBγ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y243 # IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
SIZE: 479 amino acids; 55775 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.
TISSUE SPECIFICITY: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
DOMAIN:SwissProt: Q9Y243 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight43kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Crosstide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-502) and in bulk (cat# 14-502-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-502M 04053252624551

Documentation

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Número de referencia Descripción
14-502 Akt3/PKBγ (S472D) Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases