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114700 Activin A, Human, Recombinant, CHO Cells

Activin A, Human, Recombinant, is a member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. A disulfide-linked homodimer of two 116-a.a. βA subunits.

Activin A, Human, Recombinant, CHO Cells: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
114700
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Descripción

Replacement Information

Tabla espec. clave

Purity
≥95% by SDS-PAGE

Products

Número de referenciaEmbalaje Cant./Env.
114700-10UG Ampolla de plást. 10 μg
Description
OverviewRecombinant, human activin A (disulfide-linked homodimer of two 116-amino acid residue βA subunits) expressed in CHO cells. Mature βA-monomer lacks a potential N-linked glycosylation site and has a calculated molecular weight of 13 kDa. A member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. Shown to play an important role in the regulation of hormonogenesis, cellular homeostasis, and differentiation programs. Reported to inhibit endogenous production of IL-6.
Catalogue Number114700
Brand Family Calbiochem®
References
ReferencesZipori, D., et al. 2001. J. Leukoc. Biol. 69, 867.
Hedger, M.P., et al. 2000. Cytokine 12, 595.
Woodruff, T.K. 1998. Biochem. Pharmacol. 55, 953.
Ying, S.Y., et al. 1997. Proc. Soc. Exp. Biol. Med. 214, 114.
Schwall, R.H., and Lei, C. 1991. Methods Enzymol. 198, 340.
Mason, A., et al. 1986. Biochem. Biophys. Res. Commun. 135, 957.
Product Information
FormLyophilized
FormulationLyophilized from sterile filtered 35% acetonitrile, 0.1% TFA, 50 µg BSA/µg Activin A.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.5-2.0 ng/ml as measured by its ability to induce hemoglobin expression in K562 cells
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 pg/µg Activin A
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
114700-10UG 04055977208085

Documentation

Activin A, Human, Recombinant, CHO Cells Certificados de análisis

CargoNúmero de lote
114700

Referencias bibliográficas

Visión general referencias
Zipori, D., et al. 2001. J. Leukoc. Biol. 69, 867.
Hedger, M.P., et al. 2000. Cytokine 12, 595.
Woodruff, T.K. 1998. Biochem. Pharmacol. 55, 953.
Ying, S.Y., et al. 1997. Proc. Soc. Exp. Biol. Med. 214, 114.
Schwall, R.H., and Lei, C. 1991. Methods Enzymol. 198, 340.
Mason, A., et al. 1986. Biochem. Biophys. Res. Commun. 135, 957.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2008 RFH
DescriptionRecombinant, human activin A (disulfide-linked homodimer of two 116-amino acid residue βA subunits) expressed in CHO cells. Mature βA-monomer lacks a potential N-linked glycosylation site and has a calculated molecular mass of 13 kDa. Activins are members of the TGF-β superfamily and have been shown to play an important role in the regulation of homonogenesis, cellular homeostasis, and differentiation programs.
FormLyophilized
FormulationLyophilized from sterile filtered 35% acetonitrile, 0.1% TFA, 50 µg BSA/µg Activin A.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤100 pg/µg Activin A
Biological activityED₅₀ = 0.5-2.0 ng/ml as measured by its ability to induce hemoglobin expression in K562 cells
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesZipori, D., et al. 2001. J. Leukoc. Biol. 69, 867.
Hedger, M.P., et al. 2000. Cytokine 12, 595.
Woodruff, T.K. 1998. Biochem. Pharmacol. 55, 953.
Ying, S.Y., et al. 1997. Proc. Soc. Exp. Biol. Med. 214, 114.
Schwall, R.H., and Lei, C. 1991. Methods Enzymol. 198, 340.
Mason, A., et al. 1986. Biochem. Biophys. Res. Commun. 135, 957.