Chemiluminescent Westerns

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Chemiluminescent detection uses an enzyme to catalyze a reaction that results in the production of visible light. Detection is achieved by either exposing the blot to X-ray film or acquiring the image directly in a chemiluminescence- compatible digital imaging system, usually equipped with highly-cooled CCD cameras to avoid electronic noise. Reprobing is possible with chemiluminescent substrates.

There are a variety of chemiluminescent substrates offering researchers different levels of sensitivity of detection. Traditional or low-sensitivity substrates allow protein detection at the picogram level. While these substrates may be appropriate for routine applications, they cannot detect low abundance proteins. New, high-sensitivity substrates, such as Immobilon® substrates, allow visualization of proteins at the femtogram level. However, use of these powerful substrates often requires optimization of primary and secondary antibody concentrations.

Luminata™ Western HRP Substrates

Chemiluminescent HRP (horseradish peroxidase) substrates (also known as ECL reagents) are the most sensitive reagents used in the detection of Western blots.

The Luminata™ Western HRP Substrates are a family of three premixed HRP substrates, which offer several advantages over other detection reagents. Pour directly onto the Western membrane without worrying about pipetting error. The Luminata™ Classico, Crescendo and Forte substrates cover a broad range of sensitivities.  

Advantages of Luminata™ Chemiluminescent Detection Substrates

• Premixed for convenience and to avoid cross contamination of the reagents.
• Broad range of sensitivities to satisfy all detection needs.
• Consistent results with less pipetting error.
• Stable at 4 °C or room temperature so you can store it on your own bench.
• Best detection performance relative to competition.
• Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.