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Pluripotent Mouse ES Media

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Featured Product:
ESGRO mLIF Medium Supplement
ESGRO media supplement for mouse ES culture
ESGRO supplement is a special formulation of mouse LIF protein. Unlike regular LIF, which is sold by weight, each lot of ESGRO supplement is sold based on its biological activity for reproducible results. The benefits of using ESGRO mLIF medium supplement include:
  • Consistent inhibition of ES cell differentiation
  • No batch-to-batch variation
  • Feeder-free cell culture
For certain cell lines, the use of ESGRO supplement allows feeder-free cell culture, saving time and giving you more control over your cells.


Mouse ES Media Supplements



Mouse ES Differentiation Media

Mouse ES Cell Expansion

Merck offers unique cell culture media solutions for mouse embryonic stem cell expansion which includes the following gold standard and proprietary products:

  • ESGRO mLIF: Leukemia Inhibitory Factor (LIF) is a critical growth factor that controls self-renewal and pluripotency in mouse ES cells.
  • ESGRO Complete Plus Medium: The ESGRO Complete system is the first to offer a complete medium for the serum-free and feeder-free culture of mouse ES cells.
  • ESGRO 2i Medium: A more defined version of ESGRO complete with a GSK and MEK inhibitor for better growth characteristics which has been shown to maintain mouse ES/iPS in naïve state.
Used by the most influential stem cell researchers...
  1. Yamanaka, S. et al. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation. J Cell Sci. 2004 Nov 117:5681-6.
  2. Thomson, J et al. Caspase Activity Mediates the Differentiation of Embryonic Stem Cells. Cell Stem Cell. 2008 Jun 5;2(6):595-601.
  3. Melton, D et al. Wnt signaling specifies and patterns intestinal endoderm. Mechanisms of Development. 2011 Sep-Dec;128(7-10):387-400.
  4. Gage, F et al. Maintenance of embryonic stem cell pluripotency by Nanog-mediated reversal of mesoderm specification. Nature Clinical Practice Cardiovascular Medicine. 2006 Mar;3 Suppl 1:S114-22.
  5. Gurdon, J. et al. Citrullination regulates pluripotency and histone H1 binding to chromatin. Nature. 2014 Mar 6;507(7490):104-8.
  6. Smith, A. et al. Self-renewal of teratocarcinoma and embryonic stem cells. Oncogene. 2004, 23:7150–7160.
  7. Jaenisch, R et al. Metastable Pluripotent States in NOD-Mouse-Derived ESCs. Cell Stem Cell. 2009. 5;4(6):513-24.
  8. Daley, G. et al. Ras-MAPK signaling promotes trophectoderm formation from embryonic stem cells and mouse embryos. Nature Genetics. 2008 Jul;40(7):921-6.
...in the top peer-reviewed stem cell journals. 

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