Lentiviral Biosensors |
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Lentiviral vector systems are a popular tool for introducing genes and gene products into cells. Advantages over non-viral methods (such as chemical-based transfection) include higher-efficiency transfection of dividing and non-dividing cells, stable expression of the transgene, and low immunogenicity.
Merck’s new LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure, enables visualization under different cell/disease states in live cell and in vitro analysis.
Features and Benefits:
- Pre-packaged, ready-to-use, fluorescently-tagged with monomeric GFP & RFP
- Minimum titer (≥3 x 10^8 IFU/mL) per vial
- Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods
- Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells
- Non-disruptive towards cellular function
- Validated for fluorescent microscopy and live cell analysis
Live Cell Visualization
- LentiBrite™ GFP-LC3 Lentiviral Biosensor
- LentiBrite™ EB3-GFP Lentiviral Biosensor
- LentiBrite™ RFP-EB3 Lentiviral Biosensor
- LentiBrite™ Histone H2B-RFP Lentiviral Biosensor
- LentiBrite™ Histone H2B-GFP Lentiviral Biosensor
LentiBrite GFP-LC3 Lentiviral Biosensor,
Catalogue No. 17-10193Timecourse imaging of HT-1080 cells after transduction with LentiBrite GFP-LC3 and the addition of a lysosomal inhibitor under starvation conditions. GFP-LC3 displays a diffuse nuclear and cytosolic distribution in fed cells, and a punctate distribution in starved, autophagic cells.
LentiBrite EB3-GFP Lentiviral Biosensor,
Catalogue No. 17-10208REF52 cells were plated in a chambered coverglass and transduced with lentiviral particles. Cells were imaged live by oil immersion wide-field fluorescence microscopy. The EB3-GFP displays a punctate distribution characteristic of microtubule ends.
LentiBrite RFP-EB3 Lentiviral Biosensor,
Catalogue No. 17-10222REF52 cells were plated in a chambered coverglass and transduced with lentiviral particles. Cells were imaged live by oil immersion wide-field fluorescence microscopy. The EB3-RFP displays a punctate distribution characteristic of microtubule ends.
LentiBrite Histone H2B-RFP Lentiviral Biosensor,
Catalogue No. 17-10228U2OS cells were plated in chamber slides and transduced with lentiviral particles. Cells were imaged live by oil immersion wide-field fluorescence microscopy. The cell displays metaphase chromosomes (H2B-RFP) as it transitions to anaphase.
LentiBrite Histone H2B-GFP Lentiviral Biosensor,
Catalogue No. 17-10229U2OS cells were plated in chamber slides and transduced with lentiviral particles. Cells were imaged live by oil immersion wide-field fluorescence microscopy. The cell is displaying prophase, where the chromatin (H2B-GFP) condenses into chromosomes.
Higher Efficiency Transfection Compared to Chemical or Non-viral-based Transfection Methods
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HUVECs were transfected with GFP-Tubulin plasmids using Lipofectamine 2000 (top row) or LentiBrite GFP-Tubulin lentiviral particles (bottom row). The nucleus is stained with DAPI (blue). The LentiBrite lentiviral transfection yields higher efficiency and more uniform expression of GFP-Tubulin versus chemical transfection.
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Higher Efficiency Transfection Compared to Baculovirus
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LentiBrite lentiviral particles encoding GFP-LC3 (Catalogue No. 17-10193) were adjusted to 1 mL, and equivalent volumes of lentivirus and baculovirus from a competitor product were added to HT1080 cells at an MOI of 20. Lentiviral transfection provides a stronger overall signal of GFP-LC3 compared to baculovirus transfection, when using equivalent amounts of each product.
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Ability to Transfect Dividing, Non-dividing, and Difficult-to-transfect Cell Types
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| LentiBrite Paxillin-GFP (Catalogue No. 17-10154) Primary cells (Human Mesenchymal Stem Cells) were plated in chamber slides and transduced with lentiviral particles at an MOI of 5 for 24 hours. |
LentiBrite RFP-Beta Actin (Catalogue No. 17-10203) Primary cells (HUVECs) were plated in chamber slides and transduced with lentiviral particles at an MOI of 40 for 24 hours. |
LentiBrite GFP-Vimentin (Catalogue No. 17-10152) Primary cells (Human Mesenchymal Stem Cells) were plated in chamber slide and transduced with lentiviral particles at an MOI of 10 for 24 hours. |
LentiBrite Lentiviral Biosensor Family
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More GFP- & RFP-tagged lentiviral biosensors coming soon!