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SCR713 TagGFP2 Simplicon® RNA

1) To evaluate the Simplicon® expression system in targeted cell(s) before ordering a custom Simplicon® RNA
2) To determine optimal transfection conditions to express the self-replicating RNA in cell lines

TagGFP2 Simplicon® RNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SCR713
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCR713
DescriptionTagGFP2 Simplicon® RNA
Background InformationSimplicon® is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1. The Simplicon® RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been completely removed and replaced with the transgene(s) of interest. Because Simplicon® is a synthetic RNA, there is no risk of the transgene(s) integrating into the transfected cell’s genome. Moreover, the self-replicating nature of the Simplicon™ means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon® technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc1.

The Simplicon® TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon® expression system in targeted cell(s) before ordering a custom Simplicon® RNA. Simplicon® TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon® TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
References
Product Information
Components
  • 1.
  • TagGFP2 RNA: (Part No. CS222751) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.

    2.
  • B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
Quality LevelMQ100
Applications
Application1) To evaluate the Simplicon® expression system in targeted cell(s) before ordering a custom Simplicon® RNA
2) To determine optimal transfection conditions to express the self-replicating RNA in cell lines
Application NotesThe Simplicon® TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon® expression system in targeted cell(s) before ordering a custom Simplicon® RNA. Simplicon® TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells.
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Discrete RNA band on RNA gel of appropriate size.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR713 04054839233357