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GF174 Sonic HedgeHog (Shh) Protein, Human Recombinant

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: Q15465
GF174
25 µg  
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Overview

Key Spec Table

SourcePurity
E. coli.Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue NumberGF174
DescriptionSonic HedgeHog (Shh) Protein, Human Recombinant
Alternate Names
  • SHH
  • HHG-1
Background InformationThe hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Product Information
PresentationProduct is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
ApplicationThe hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.
Biological Information
PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
SourceE. coli.
Cross ReactivityNone
UniProt Number
Product Usage Statements
Quality AssuranceBioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size25 µg
Global Trade Item Number
Catalogue Number GTIN
GF174 04053252691393