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HTS174RTA Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells

Human secretin receptor / SEC GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_002980
HTS174RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1secretin receptor / SEC
Description
Catalogue NumberHTS174RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ Secretin Glucagon Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Secretin, a member of the secretin-glucagon peptide hormone family, is a 27 amino acid peptide that was originally isolated from the duodenum. In the duodenum, secretin is released to stimulate the release of digestive juices in the pancreas (Bayliss et al, 1902). The receptor for secretin is a class 2 (or class B) G protein coupled receptor that signals through Gs to stimulate cAMP production (Dong et al, 2002). Along with its traditional role in the pancreas, studies in secretin-deficient mice have shown miscommunication between the CA3 Schaffer collateral and CA1 pyramidal neurons, causing a deficiency in synaptic transmission (Nishijima et al, 2006). This miscommunication of CA1 dendrites is found in Autism, Rett Syndrome, and most forms of mental retardation, suggesting secretin could be a potential target for treatment of these disorders. Millipore’s cloned human Secretin-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Secretin expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at Secretin.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman secretin receptor / SEC GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
Key Applications
  • Calcium Flux Assays
  • cAMP Assays
Biological Information
Entrez Gene Number
GPCR ClassB
Host CellsChem-1
Protein Targetsecretin receptor / SEC
Target Sub-FamilyGlucagon
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS174RTA 04053252630040

Documentation

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Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells