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559309 PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

This PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
559309
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Ch, Dr, H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
559309-10T Plastic ampoule 10 t
Description
OverviewRecognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
  • Antibody Target Gene Symbol: MAPK8, MAPK9
  • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
  • Entrez Gene Name: mitogen-activated protein kinase 8
  • Hu Entrez ID: 5601
  • Mu Entrez ID: 26420
  • Rat Entrez ID: 50658
    		•
    Catalogue Number559309
    Brand Family Calbiochem®
    Application Data
    Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
    References
    ReferencesFanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.
    Product Information
    FormLiquid
    FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
    Preservative≤0.1% sodium azide
    Quality LevelMQ100
    Applications
    Key Applications Immunoblotting (Western Blotting)
    Immunohistochemistry
    Application NotesImmunoblotting (1:1000)
    Immunohistochemistry (see comments)
    Application CommentsSpecific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
    Biological Information
    Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Species Reactivity
    • Nematode
    • Chicken
    • Drosophila
    • Human
    • Mouse
    • Rat
    Antibody TypePolyclonal Antibody
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Standard Handling
    Storage ≤ -70°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    559309-10T 04055977192445

    Documentation

    PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb SDS

    Title

    Safety Data Sheet (SDS) 

    PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb Certificates of Analysis

    TitleLot Number
    559309

    References

    Reference overview
    Fanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision18-June-2009 JSW
    ApplicationImmunoblotting (1:1000)
    Immunohistochemistry (see comments)
    Application Data
    Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
    DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
    BackgroundC-Jun-N-terminal kinase (JNK), also called stress-activated protein kinase (SAPK) is a member of the mitogen activated protein kinase (MAPK) family. JNK is activated in response to various extracellular stimuli, including pro-inflammatory cytokines. Phosphorylation of threonine and tyrosine in the Thr-Pro-Tyr domain is required for full activation. Mitogen activated protein kinase kinase (MKK4) preferentially phosphorylates tyrosine 185, and MKK7 causes preferential phosphorylation of threonine 183.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
    IsotypeIgG
    SpeciesC. elegans, Drosophila, chicken, human, mouse, rat
    FormLiquid
    FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
    Preservative≤0.1% sodium azide
    CommentsSpecific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesFanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.