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407707 PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

407707
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
407707-10T Plastic ampoule 10 t
Description
OverviewRecognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Catalogue Number407707
Brand Family Calbiochem®
Application Data
Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
References
ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Schmid, E., at al. 1998. FASEB J. 12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ebina, Y., et al. 1985. Cell 40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Application CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
407707-10T 04055977211474