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14-603 PI3 Kinase (p110β/p85α) Protein, active, 20 µg

Active complex of N-terminal His6-tagged recombinant full-length human p110β & untagged, recombinant, full length, human p85&alpha. For use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-603
20 µg  Also available in 250μg size as catalog # 14-603M - Call for pricing and availability.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-603
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI3 Kinase (p110β/p85α) Protein, active, 20 µg
OverviewComplex of N-terminal His6-tagged recombinant full-length human p110β and untagged, recombinant, full length, human p85&alpha
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of N-terminal His6-tagged recombinant full-length human p110β & untagged, recombinant, full length, human p85&alpha. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCoexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]
Gene Symbol
  • PIK3CB
  • DKFZp779K1237
  • PI3K
  • p110-BETA
  • PI3Kbeta
  • MGC133043
  • PIK3C1
Protein TargetPI3Kβ/p85α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1070 amino acids; 122762 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Molecular Weightp110β (124kDa) & p85&alpha (83.7kDa)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated in a 30 minute reaction using the PIProfiler™ protocol (see www.upstate.com/features/piprofiler).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size20 µg
Material PackageAlso available in 250μg size as catalog # 14-603M - Call for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-603 04053252326431

Documentation

PI3 Kinase (p110β/p85α) Protein, active, 20 µg SDS

Title

Safety Data Sheet (SDS) 

PI3 Kinase (p110β/p85α) Protein, active, 20 µg Certificates of Analysis

TitleLot Number
PI3 Kinase (p110#946;) - 1961292-A 1961292-A
PI3 Kinase (p110#946;) - 1961292-B 1961292-B
PI3 Kinase (p110#946;) - 1961292-C 1961292-C
PI3 Kinase (p110#946;) - 1994929-A 1994929-A
PI3 Kinase (p110#946;) - 1994929-B 1994929-B
PI3 Kinase (p110#946;/p85#945;), active - 1961292-E 1961292-E

Technical Info

Title
Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

Data Sheet

Title
PI 3-Kinase HTRF Assay

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-603M PI3 Kinase (p110β/p85α) Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases