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70666 Ni-NTA His•Bind® Resin

70666
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70666-3 Plastic ampoule 10 ml
70666-4 Glass bottle 25 ml
70666-5 Glass bottle 100 ml
Description
OverviewNi-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind® Resin has a binding capacity of 5–10 mg His•Tag® fusion protein per ml resin.

Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.
Catalogue Number70666
Brand Family Novagen®
References
Product Information
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 10-22-42/43

Flammable.
Harmful if swallowed.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36-7

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Keep container tightly closed.
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Flammable
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70666-3 07790788054946
70666-4 07790788052768
70666-5 04055977334074

Documentation

Ni-NTA His•Bind® Resin SDS

Title

Safety Data Sheet (SDS) 

Ni-NTA His•Bind® Resin Certificates of Analysis

TitleLot Number
70666

Brochure

Title
Protein Purification and Detection Tools

Technical Info

Title
Ni-NTA HisユBind® Resins

Citations

Title
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  • Aileen Aherne, et al. (2004) On the molecular patholo
  • User Protocols

    Title
    TB273 Ni-NTA His•Bind® Resin