530838 Sigma-AldrichNMD Inhibitor - CAS 307519-88-6 - Calbiochem
A cell-permeable inhibitor of nonsense-mediated mRNA decay (NMD) that enhances the stability of premature termination codon mutated p53 mRNA in N417 and HDQP-1 cells.
More>> A cell-permeable inhibitor of nonsense-mediated mRNA decay (NMD) that enhances the stability of premature termination codon mutated p53 mRNA in N417 and HDQP-1 cells. Less<<MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Synonyms: Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14
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Overview
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Key Spec Table
| CAS # | Empirical Formula |
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| 307519-88-6 | C₂₁H₂₅N₃O₄S |
Products
| Catalogue Number | Packaging | Qty/Pack | |
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| 5308380001 | Glass bottle | 10 mg |
| Description | |
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| Overview | A cell-permeable tetrahydroquinoxalinyl-thiophenecarboxylate compound that inhibits nonsense-mediated mRNA decay (NMD) in a dose-dependent manner and enhances the stability of premature termination codon (PTC) mutated p53 mRNA in N417 and HDQP-1 cells. However, it does not affect the stability of wild-type p53 in U2OS cells. Shown to dock reversibly within a SMG7 pocket and disrupt SMG7-UPF1 interaction and prevent their complex formation. Acts synergistically with G418 (Cat. No. 345810; 200 µg/ml) to increase p53 expression in p53 PTC mutant cells leading to increase mRNA levels of p21, Bax, and PUMA. Does not display any toxicity (> 50 µM) in multiple cell lines and has no effect on proliferation and protein synthesis in transformed and non-transformed cells. Please note that the molecular weight for this compound is batch-specific due to variable water content. |
| Catalogue Number | 530838 |
| Brand Family | Calbiochem® |
| Synonyms | Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14 |
| References | |
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| References | Martin, L., et al. 2014. Cancer Res. 74, 3104. |
| Product Information | |
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| CAS number | 307519-88-6 |
| Form | Yellow powder |
| Hill Formula | C₂₁H₂₅N₃O₄S |
| Chemical formula | C₂₁H₂₅N₃O₄S |
| Reversible | Y |
| Quality Level | MQ100 |
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| Biological Information | |
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| Primary Target | SMG7 |
| Purity | ≥95% by HPLC |
| Physicochemical Information | |
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| Cell permeable | Y |
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| Packaging Information | |
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| Packaged under inert gas | Packaged under inert gas |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 5308380001 | 04055977243000 |
Documentation
NMD Inhibitor - CAS 307519-88-6 - Calbiochem SDS
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References
| Reference overview |
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| Martin, L., et al. 2014. Cancer Res. 74, 3104. |
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Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
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