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SCM008 Mouse Neural Stem Cell Expansion Medium

SCM008
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCM008
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionMouse Neural Stem Cell Expansion Medium
OverviewThe Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
Materials Required but Not Delivered• Cryopreserved Mouse Cortical Neural Stem Cells (Millipore Catalog No. SCR029)
• Cryopreserved Mouse Spinal Cord Neural Stem Cells (Millipore Catalog No. SCR031)
• Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
• Poly-L-ornithine (Sigma Catalog No. P3655)
• Laminin (Millipore Catalog No. CC095)
• Accutase solution (Millipore Catalog No. SCR005)
References
Product Information
Components
  • 500 mL of Neural Stem Cell Basal Medium (Cat.# SCM003)
  • bFGF, 10 ug
  • EGF, 10 ug
  • Heparin, 1.5 mg
HS Code3821 00 00
Quality LevelMQ100
Applications
ApplicationThe Mouse Neural Stem Cell Expansion Medium allows for the growth & proliferation of mouse neural stem cells.
Key Applications
  • Stem Cell Culture
Biological Information
Media FormLiquid
Stem Cell Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPart number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.


FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw.


bFGF, EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCM008 04053252662485

Documentation

Mouse Neural Stem Cell Expansion Medium SDS

Title

Safety Data Sheet (SDS) 

Mouse Neural Stem Cell Expansion Medium Certificates of Analysis

TitleLot Number
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 2470945
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 3028341
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 3129111
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 3004416
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 3119511
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 2924830
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 2926280
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 3085225
MOUSE NEURAL STEM CELL EXPANSION MEDIUM 2879060
MOUSE NEURAL STEM CELL EXPANSION MEDIUM - 2362926 2362926