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113725 HPTLC Silica gel 60 RP-8 F₂₅₄s

113725
Purchase on Sigma-Aldrich

Overview

Replacement Information
Replacement InformationIn North America 1.13725.0001 replaces and is identical to 13725-5

Products

Catalogue NumberPackaging Qty/Pack
1137250001 Fibre case 25 units
Description
Catalogue Number113725
Replaces13725-5; 13725
DescriptionHPTLC Silice gel 60 RP-8 F₂₅₄s
OverviewRP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
References
Product Information
HS Code3822 19 90
Quality LevelMQ100
Applications
ApplicationGlass HPTLC Silice gel 60 RP-8 F₂₅₄s plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Specific surface area (according to BET; 5-Pt. measurement)480 - 540 m²/g
Pore volume (N₂-isotherm)0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution)5 - 7 µm
Layer thickness150 - 200 µm
Deviation of layer thickness per plate≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test

48 - 60
B) Steroid test
- methyltestosterone, steroid test31 - 51
- Reichstein S, steroid test59 - 79
- hydrocortisone, steroid test72 - 92
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)
Global Trade Item Number
Catalogue Number GTIN
1137250001 04022536151175

Documentation

HPTLC Silica gel 60 RP-8 F₂₅₄s SDS

Title

Safety Data Sheet (SDS) 

HPTLC Silica gel 60 RP-8 F₂₅₄s Certificates of Analysis

Product Number PackagingSpecificationLot Number
1137250001 Fibre casePDF Specification Document

Brochure

Title
Fast and Precise. Thin Layer Chromatography by.

Technical Info

Title
Separation of aromatic carboxylic acids
Separation of digested proteins on Silica gel 60 RP-8
Separation of peptides
Separation of steroids
Separation of stilbestrol derivatives
Separation of tri-peptides
Separation of tri-peptides
Separation of tri-peptides

Related Products & Applications

Alternative Products

Catalogue Number Description
115388 TLC Silica gel 60 RP-8 F₂₅₄s
115424 TLC Silica gel 60 RP-8 F₂₅₄s
115684 TLC Silica gel 60 RP-8 F₂₅₄s

Related Products

Catalogue Number Description
115388 TLC Silica gel 60 RP-8 F₂₅₄s
115424 TLC Silica gel 60 RP-8 F₂₅₄s
115684 TLC Silica gel 60 RP-8 F₂₅₄s

Accessories (Primary)

Catalogue Number Description
112537 UV lamp 254 nm

Related Products By: Application Facete

Categories

Analytics and Sample Preparation > Analytical Chromatography > Thin-Layer Chromatography (TLC) Products > High Performance Thin-Layer Chromatography Plates (HPTLC) > RP-modified Silica Plates