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AP501A Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed

Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Mouse
AP501A
1 mL  
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Description
Catalogue NumberAP501A
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed
Background InformationIgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Product Information
FormatAlkaline Phosphatase
PresentationPurified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
Quality LevelMQ100
Applications
ApplicationGoat anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA.
Key Applications
  • ELISA
Application NotesELISA: 1:2,000-1:4,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPrepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
EpitopeMouse IgM, IgG, IgA
HostGoat
SpecificityThe antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Species Reactivity
  • Mouse
Species Reactivity NoteReacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
Antibody TypePolyclonal Antibody
Purification MethodAffinity Chromatography
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long term at -20°C.
Packaging Information
Material Size1 mL
Global Trade Item Number
Catalogue Number GTIN
AP501A 04053252269790