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AP124JA4 Goat Anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate Antibody

AP124JA4
1.5 mg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information
Description
Catalogue NumberAP124JA4
Trade Name
  • Chemicon
DescriptionGoat Anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate Antibody
Background InformationAlexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
References
Product Information
FormatAlexaFluor®488
PresentationLyophilized in buffer containing 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA (IgG-Free, Protease-Free) and 0.05% sodium azide.
Applications
ApplicationThis Goat anti-Mouse IgG (H+L), Alexa Fluor 488 conjugate Antibody is validated for use in Immunofluorescence for the detection of Mouse IgG (H+L).
Key Applications
  • Immunofluorescence
Application NotesReconstitute the product to 1.5 mg/mL with dH2O.
Suggested dilution range for most applications: 1:100 – 1:800.
Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenFluorophore Information
Alexa Fluor® 488 carboxylic acid
Amax=493; Emax=519
Fluorophore/Protein Absorbance Ratio: A493/A280=1.9
Concentration1.5 mg
HostGoat
SpecificityBased on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Species Reactivity
  • Mouse
Species Reactivity NoteThe antibody may cross-react with immunoglobulins from other species.
Antibody TypePolyclonal Antibody
Purification MethodThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC lyophilized from date of receipt.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size1.5 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP124JA4 04053252693830

Documentation

Goat Anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate Antibody Certificates of Analysis

TitleLot Number
Goat anti-Mouse IgG (H+L), Alexa Fluor#174; 488 conjugate - 2116696 2116696
Goat anti-Mouse IgG (H+L), Alexa Fluor#174; 488 conjugate - 2148306 2148306
Goat anti-Mouse IgG (H+L), Alexa Fluor#174; 488 conjugate - 2189747 2189747
Goat anti-Mouse IgG (H+L), Alexa Fluor#174; 488 conjugate - 2205027 2205027
Goat anti-Mouse IgG (H+L), Alexa Fluor#174; 488 conjugate - Q2027082 Q2027082