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12-349 Goat Anti-Mouse IgG Antibody, HRP conjugate

12-349
500 µg  
Purchase on Sigma-Aldrich

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Overview

Description
Catalogue Number12-349
Brand Family Upstate
Trade Name
  • Upstate
DescriptionGoat Anti-Mouse IgG Antibody, HRP conjugate
Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
FormatHRP
PresentationPurified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Quality LevelMQ100
Applications
ApplicationGoat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application NotesAdditional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
Biological Information
ImmunogenHighly purified whole mouse IgG.
HostGoat
SpecificityRecognizes mouse IgG, both heavy and light chains.
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Product Usage Statements
Quality AssuranceWestern Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size500 µg
Global Trade Item Number
Catalogue Number GTIN
12-349 04053252278358