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322326 Diphtheria Toxin, Unnicked, Corynebacterium diphtheriae - Calbiochem

Diphtheria toxin catalyzes ADP-ribosylation of eukaryotic aminoacyltransferase II (EF2) using NAD as substrate. Activation requires nicking with a protease followed by reduction with DTT.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
322326
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Overview

Products

Catalogue NumberPackaging Qty/Pack
322326-1MG Glass bottle 1 mg
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Description
OverviewCatalyzes ADP-ribosylation of eukaryotic aminoacyltransferase II (EF2) using NAD as substrate, thereby inhibiting protein synthesis. May also induce internucleosomal breakdown. Causes DNA fragmentation and cytolysis in U937 cells. Activation requires nicking with a protease followed by reduction with DTT.
Catalogue Number322326
Brand Family Calbiochem®
References
ReferencesKochi, S.K., and Collier, R.J. 1993. Exp. Cell Res. 208, 296.
Chang, M.P., et al. 1989. J. Biol. Chem. 264, 15261.
Pappenheimer, A.M., Jr. 1977. Annu. Rev. Biochem. 46, 69.
Ittelson, T.R., and Gill, D.M. 1973. Nature 242, 330.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, A.M., et al. 1972. Immunochem. 9, 891.
Uchida, T., et al. 1972. Science 175, 901.
Bowman, C.G., and Bonventre, P.F. 1970. J. Exp. Med. 131, 659.
Baseman, J.B., et al. 1970. J. Exp. Med. 132, 1138.
Gill, D.M., et al. 1969. J. Exp. Med. 129, 1.
Gill, D.M., et al. 1969. Cold Spring Harbor Symp. Quant. Biol. 34, 589.
Honjo, J., et al. 1968. J. Biol. Chem. 243, 3553.
Product Information
ATP CompetitiveN
FormSolid
FormulationLyophilized from sterile 10 mM Tris, 1 mM EDTA, pH 7.5.
ReversibleN
Quality LevelMQ100
Biological Information
Biological activityNote: Toxicity may vary by lot of toxin. Each laboratory should determine the optimum dosage for each particular application.
Primary TargetEukaryotic aminoacyltransferase II (EF2)
PurityMajor band (under reducing conditions) of ~63 kDa
Physicochemical Information
Cell permeableN
Safety Information according to GHS
RTECSXW5807200
Safety Information
R PhraseR: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S PhraseS: 36

Wear suitable protective clothing.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, quickly freeze on dry ice, and freeze (-70°C). Subsequent thawing should be carried out only at room temperature. For assays employing very low concentrations of diphtheria toxin, the use of a carrier protein, such as BSA or HSA, is recommended.
Global Trade Item Number
Catalogue Number GTIN
322326-1MG 07790788060923