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428901 Cholesterol/Cholesteryl Ester Quantitation Kit

428901
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
428901-1KIT Glass bottle 1 kit
Description
OverviewA sensitive assay for the quantitative measurement of cholesterol and/or cholesteryl ester by a colorimetric or a fluorometric method. Measurement by spectrophotometry at OD =570 nm or fluorometry at Ex/Em =535/587 nm. This assay can measure cholesterol itself, total cholesterol (cholesterol + cholesterol ester) by adding cholesterol esterase to the reaction, or only cholesteryl ester by subtracting the value of cholesterol from the total value of cholesterol plus cholesteryl esters. The fluorometric assay can detect 0.02-1 µg/well and is 4-10 fold more sensitive than colorimetric assay. Kit contains sufficient reagents for 100 tests.
Catalogue Number428901
Brand Family Calbiochem®
Materials Required but Not Delivered Spectrophotometer capable of reading absorbance at 570 nm or a fluorimeter capable of measuring fluorescence at an excitation wavelength of ~535 nm and an emission wavelength of ~587 nm
96-well plate
Chloroform-NP-40 (1% NP-40 in pure chloroform) (for preparation of cell lysates and tissue extracts)
Product Information
Form100 Tests
Format96-well plate
Kit containsCholesterol Reaction Buffer, Cholesterol Probe, Dimethylsulfoxide, Enzyme Mix, Cholesterol Esterase, Cholesterol Standard, and a user protocol.
Quality LevelMQ100
Biological Information
Assay range0.2-10 µg/well
Sample TypeCells, tissues, serum
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
Protect from Light Protect from light
Do not freeze Ok to freeze
Supplemental Information
Kit containsCholesterol Reaction Buffer, Cholesterol Probe, Dimethylsulfoxide, Enzyme Mix, Cholesterol Esterase, Cholesterol Standard, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
428901-1KIT 04055977210118