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SCT057 BioTracker ERthermAC Temperature-Sensitive Live Cell Dye

Live cell imaging thermosensitive dye to visualize heat production in brown adipocytes.

SCT057
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT057
DescriptionBioTracker ERthermAC Temperature-Sensitive Live Cell Dye
OverviewBrown adipocyte tissue (BAT) has been shown to be present in human newborns, small infants, as well as adults. Stimulation of BAT energy expenditure is considered a potential new drug target for obesity and diabetes treatments, and there is increasing interest in the discovery of novel mechanisms and substances for this purpose. However, to facilitate the development of breakthrough therapies, it is essential that techniques to measure the signature function of BAT are readily available.

The BioTracker ERthermAC thermosensitive live cell dye is a novel BODIPY-based dye that is capable of imaging heat production in individual brown adipocytes. The dye’s features such as small size, excellent cellular uptake, low cytotoxicity and robust photostability make it very biocompatible. Data obtained using this dye is comparable with that obtained by conventional methods measuring mitochondrial depolarization, oxygen consumption, and extracellular acidification rates, and with thermal power measurements obtained using the calScreener.

Spectral Properties
Absorption: 565 nm
Emissions: 590 nm (Orange/Red) in polarized mitochondria
Emissions: 527 nm (Green) in depolarized mitochondria

References
Kriszt R et al. Optical visualization of thermogenesis in stimulated single-cell brown adipocytes. Sci Rep. 2017 May 3;7(1):1383.
Alternate Names
  • Live cell imaging probe
References
Product Information
Detection methodFluorescence
HS Code3204 90 00
PresentationLyophilized
Applications
ApplicationLive cell imaging thermosensitive dye to visualize heat production in brown adipocytes.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight541.28 g/mol
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 541.28 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker ERthermAC Temperature-sensitive Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT057 04054839008382