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05-1059 Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases, Inflammation & Autoimmune Mechanisms Gene Symbol: P38B, P38BETA2, PRKM11, SAPK2 , SAPK2B, p38-2, p38Beta, p38b UniProt Number: Q15759
05-1059
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, VrtWB, IFRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number05-1059
DescriptionAnti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal
Alternate Names
  • CSAID-binding protein
  • Csaids binding protein
  • Cytokine suppressive anti-inflammatory drug-binding protein
  • MAP kinase MXI2
  • MAP kinase p38 alpha
  • MAX-interacting protein 2
  • Mitogen-activated protein kinase p38 alpha
  • cytokine suppressive anti-inflammatory drug binding protein
  • mitogen-activated protein kinase 14
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • p38alpha Exip
  • stress-activated protein kinase 2A
Background InformationThe p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.
References
Product Information
FormatCulture Supernatant
HS Code3002 15 90
PresentationRabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.
Key Applications
  • Western Blotting
  • Immunofluorescence
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.
EpitopePhosphorylated Thr180/Tyr182
Clone8.78.8
HostRabbit
SpecificityThis antibody detects p38α when phosphorylated on Thr180/Tyr182.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Vertebrates
Species Reactivity NoteHuman and Mouse tested. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
Gene Symbol
  • P38B
  • P38BETA2
  • PRKM11
  • SAPK2
  • SAPK2B
  • p38-2
  • p38Beta
  • p38b
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium By similarity.
ENZYME REGULATION: Activated by phosphorylation on threonine and tyrosine by MKK6. Inhibited by pyridinyl-imidazole related compounds.
TISSUE SPECIFICTY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme Probable.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Molecular Weight38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1059 04053252327872

Documentation

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-p38a (Thr180/Tyr182), clone 8.78.8 - 1991289 1991289
Anti-phospho-p38a (Thr180/Tyr182), clone 8.78.8 - 2197255 2197255
Anti-phospho-p38a (Thr180/Tyr182), clone 8.78.8 - NG1651161 NG1651161
Anti-phospho-p38a (Thr180/Tyr182), clone 8.78.8 - NG1686569 NG1686569
Anti-phospho-p38a (Thr180/Tyr182), clone 8.78.8 - NG1829857 NG1829857
Anti-phospho-p38α (Thr180/Tyr182), clone 8.78.8 - 2395702 2395702
Anti-phospho-p38α (Thr180/Tyr182), clone 8.78.8 - 2437346 2437346
Anti-phospho-p38α (Thr180/Tyr182), -2585819 2585819
Anti-phospho-p38α (Thr180/Tyr182), -2723562 2723562
Anti-phospho-p38α (Thr180/Tyr182), clone 8.78.8 - 3050676 3050676
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