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FCMAB110P Sigma-AldrichAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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Research Category: Epigenetics & Nuclear Function, Apoptosis & Cancer Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ATM, AT, TELO1, ATC, DKFZp781A0353, MGC74674, ATD, ATA, AT1, T-PLL, ATE, TEL1, ATDC, TPLL UniProt Number: Q13315

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	FC	M	Phycoerythrin	Monoclonal Antibody

Description
Catalogue Number	FCMAB110P
Description	Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate
Overview	N-terminal c-Myc, GST-tagged, recombinant human Cardiac Troponin I full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Alternate Names	A-T, mutated AT mutated TEL1, telomere maintenance 1, homolog ataxia telangiectasia mutated ataxia telangiectasia mutated (includes complementation groups A, C and D) ataxia telangiectasia mutated protein human phosphatidylinositol 3-kinase homolog serine-protein kinase ATM
Background Information	Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

References

Product Information
Format	Phycoerythrin
HS Code	3002 15 90
Control	Irradiated HeLa cells
Presentation	Purified mouse monoclonal IgG1κ conjugated to phycoerythrin in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Quality Level	MQ100

Applications
Application	Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate detects level of phospho-ATM (Ser1981) & has been published & validated for use in FC.
Key Applications	Flow Cytometry

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to human ATM phosphorylated at Ser1981.
Epitope	Phosphorylated at and around Ser1981
Clone	10H11.E12
Host	Mouse
Specificity	Antibody recognizes ATM phosphorylated at Ser1981.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human and mouse. Predicted to cross-react with rat based on sequence homology
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000042
Entrez Gene Summary	The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	ATM AT TELO1 ATC DKFZp781A0353 MGC74674 ATD ATA AT1 T-PLL ATE TEL1 ATDC TPLL
Modifications	Phosphorylation
Purification Method	Protein G Purified
UniProt Number	Q13315
UniProt Summary	FUNCTION: SwissProt: Q13315 # Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. SIZE: 3056 amino acids; 350644 Da SUBUNIT: Exists in monomeric and tetrameric state. Binds DNA ends, p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. Interacts with EEF1E1. This interaction, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. Interacts with DCLRE1C. Interacts with MYST1. Interacts with HTATIP. SUBCELLULAR LOCATION: Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. TISSUE SPECIFICITY: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.DOMAIN:SwissProt: Q13315 The FATC domain is required for interaction with HTATIP. PTM: Phosphorylated by ARK5. Autophosphorylated on Ser-1981 upon DNA damage. & Acetylated by HTATIP upon DNA damage; which is required for autophosphorylation and subsequent activation. DISEASE: SwissProt: Q13315 # Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation. & Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients. & Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL). & Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure. SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 1 PI3K/PI4K domain.
Molecular Weight	~370 kDa Calculated

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry with HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Packaging Information
Material Size	100 tests

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
FCMAB110P	04053252405143

Documentation

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate SDS

Title
Safety Data Sheet (SDS)

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate Certificates of Analysis

Title	Lot Number
Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE conjugate - NRG1677934	NRG1677934
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 1990434	1990434
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2002202	2002202
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2029175	2029175
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - 2204976	2204976
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1741009	NG1741009
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1818624	NG1818624
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1871120	NG1871120
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1895075	NG1895075
Milli-Mark Anti-phospho-ATM (Ser1981), clone 10H11.E12 PE - NG1947164	NG1947164

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Categories

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