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06-573 Anti-iNOS/NOS II Antibody, NT

06-573
200 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, B, Sh, GpIHC, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number06-573
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-iNOS/NOS II Antibody, NT
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Alternate Names
  • Hepatocyte NOS 3
  • Inducible NO synthase 3
  • Inducible NOS 3
  • NOS type II 3
  • NOS, type II 2
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, macrophage 2
Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Product Information
FormatPurified
Control
  • INFγ and LPS treated L929B cell lysate, cytokine stimulated A549 cells, cytokine stimulated rat fibroblasts
PresentationPurified IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen solution.
Applications
ApplicationThis Anti-iNOS/NOS II Antibody, NT is validated for use in IH, IP, WB for the detection of iNOS/NOS II.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot of this antibody has been reported to detect iNOS/NOS II in 2% paraformaldehyde-fixed sheep lung (Sherman, T.S., 1999).

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated iNOS/NOS II from 500 μg of L929B cells which had been stimulated with 10 ng/mL IFNg and 1 μg/mL LPS.
Biological Information
ImmunogenPartial fusion protein raised against the N-terminus of murine iNOS/NOS II.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes N-terminus of iNOS, Mr 125 kDa. Does not cross-react with nNOS/NOS I.
IsotypeIgG
Species Reactivity
  • Mouse
  • Bovine
  • Sheep
  • Guinea Pig
Species Reactivity NoteMouse, bovine, sheep and guinea pig. Other species cross-reactivity is unknown.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Gene Symbol
  • EC 1.14.13.39
  • HEP-NOS
  • INOS
  • NOS
  • NOS2A
  • iNOS
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
SIZE: 1153 amino acids; 131117 Da
SUBUNIT: Homodimer. Binds SLC9A3R1.
TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Molecular Weight125 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot on lysates from L929B cells that had been stimulated IFNγ and LPS.

Western Blot Analysis: 1-4 μg/mL of this lot detected iNOS/NOS II in RIPA lysates from RAW 264.7 cells that had been stimulated with LPS. 2 μg/mL of a previous lot detected iNOS/NOS II from similarly treated L8 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size200 µg
Global Trade Item Number
Catalogue Number GTIN
06-573 08436037125461