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MAB1945 Anti-Vitronectin Antibody, clone BV2

MAB1945
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IP, WB, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1945
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Vitronectin Antibody, clone BV2
Alternate Names
  • Serum spreading factor
  • S-protein
Product Information
FormatPurified
PresentationPurified immunoglobulin by Protein A chromatography. Liquid in PBS containing 0.05% sodium azide and 0.1% BSA.
Quality LevelMQ100
Applications
ApplicationAnti-Vitronectin Antibody, clone BV2 is an antibody against Vitronectin for use in ELISA, FC, IP, WB, IH.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
  • Affects Function
Application NotesELISA

Immunoprecipitation

Western blot

Immunohistochemistry/immunofluorescence

Inhibits endothelial cell adhesion and spreading on vitronectin-coated surfaces.

Flow Cytometry

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified human plasma vitronectin (Preissner, 1985).
CloneBV2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityHuman vitronectin. Specificity determined by ELISA and immunoprecipitation of I125 labeled vitronectin purified from plasma (Zanetti et al., 1994). The antibody reacts with vitronectin both in its native and denatured form.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Gene Symbol
  • VTN
  • S-protein
  • epibolin
  • VNT
  • VN
  • V75
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
SIZE: 478 amino acids; 54306 Da
SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Plasma.
DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB1945 04053252317453