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GR08L Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)

This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TR, Anti-CD71

GR08L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GR08L-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
Catalogue NumberGR08L
Brand Family Calbiochem®
SynonymsAnti-TR, Anti-CD71
References
ReferencesSchneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
Omary, M.B., et al. 1980. Nature 286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Positive controlMost cell lines or placental tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.
Key Applications Flow Cytometry
Immunofluorescence
Immunoprecipitation
Not Western Blot
Application NotesFlow Cytometry (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (not recommended)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenK562 cells
ImmunogenHuman
CloneT56/14
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR08L-100UG 04055977228199

Documentation

Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) SDS

Title

Safety Data Sheet (SDS) 

Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) Certificates of Analysis

TitleLot Number
GR08L

References

Reference overview
Schneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
Omary, M.B., et al. 1980. Nature 286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-October-2010 RFH
SynonymsAnti-TR, Anti-CD71
ApplicationFlow Cytometry (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.
BackgroundTransferrin is the major iron binding protein in human plasma and is bound by a specific cell surface receptor during transport of iron into various tissues. The human transferrin receptor was originally isolated from placenta and shown to contain a subunit with an apparent molecular weight of 90,000. Further structural characterization of the receptor revealed that it resides in the plasma membrane as a 180,000 dimer, has a pI of 5.2, is phosphorylated predominantly on serine residues and contains both complex and high mannose oligosaccharide chains. Transferrin receptor is considered to be a marker for proliferating cells and its levels can be drastically reduced by agents which cause differentiation of undifferentiated cells such as HL-60 cells. Since many of the enzymes of metabolically active, proliferating cells require iron to catalyze redox reactions it is not surprising that the transferrin receptor would be a marker of proliferation. Further support for this hypothesis has been obtained by using antibodies to block transferrin binding and cause the accumulation of cells in S-phase, presumably due to interference with normal iron uptake and energy metabolism. Thus, monoclonal antibodies recognizing the transferrin receptor are not only valuable tools for studying iron metabolism, but may also be useful for monitoring and possible therapy of diseases involving highly proliferative cells.
HostMouse
Immunogen speciesHuman
ImmunogenK562 cells
CloneT56/14
IsotypeIgG₁
Specieshuman
Positive controlMost cell lines or placental tissue
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSchneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
Omary, M.B., et al. 1980. Nature 286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
Application referencesOriginal Clone Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.