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GF53 Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043)

This Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043) is validated for use in ELISA, Immunoblotting for the detection of Tissue Plasminogen Activator (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
GF53
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GF53-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~60 kDa tPA protein.
Catalogue NumberGF53
Brand Family Calbiochem®
Application Data
Detection of human tissue plasminogen activator by immunoblotting. Sample: Tissue from human plasminogen activator protein. Primary antibody: Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043) (Cat. No. GF53) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesHartleib, J., et al. 2000. Blood 96, 2149.
Wiman, B., et al. 2000. Arterioscler Thromb. Vasc. Biol. 20, 2019.
Pourtau, J., et al. 1998. Blood Coagul Fibrinolysis 9, 609.
Van, D.E., et al. 1995. Blood 85, 3510.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, 1% mannitol, pH 7.4.
Positive controlHuman tPA
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified human tissue plasminogen activator (tPA)
ImmunogenHuman
CloneGMA-043
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF53-100UG 04055977220148

Documentation

Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043) SDS

Title

Safety Data Sheet (SDS) 

Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043) Certificates of Analysis

TitleLot Number
GF53

References

Reference overview
Hartleib, J., et al. 2000. Blood 96, 2149.
Wiman, B., et al. 2000. Arterioscler Thromb. Vasc. Biol. 20, 2019.
Pourtau, J., et al. 1998. Blood Coagul Fibrinolysis 9, 609.
Van, D.E., et al. 1995. Blood 85, 3510.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2007 RFH
ApplicationELISA (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human tissue plasminogen activator by immunoblotting. Sample: Tissue from human plasminogen activator protein. Primary antibody: Anti-Tissue Plasminogen Activator (Ab-1) Mouse mAb (GMA-043) (Cat. No. GF53) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa tPA protein.
BackgroundEpidemiological studies have demonstrated that levels of plasma fibrinogen, von Willebrand factor (vWf), plasminogen activator inhibitor-1 (PAI-1) and tissue-type plasminogen activator (tPA) are associated with the incidence of vascular disease. Von Willebrand factor (vWF) is a large, multimeric glycoprotein mediating platelet adhesion at sites of endothelial damage. The process of acute release of tissue-type plasminogen activator (tPA) is important in locally speeding up fibrinolysis. Acutely released tPA and constitutively secreted tPA were liberated from different pathways in human umbilical vein endothelial cells. Plasma levels of tissue plasminogen activator to plasminogen activator/ inhibitor-1 complex, in addition to von Willebrand factor, are significant risk markers for recurrent myocardial infarction.
HostMouse
Immunogen speciesHuman
Immunogenpurified human tissue plasminogen activator (tPA)
CloneGMA-043
IsotypeIgG₁
Specieshuman
Positive controlHuman tPA
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, 1% mannitol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
Toxicity Standard Handling
ReferencesHartleib, J., et al. 2000. Blood 96, 2149.
Wiman, B., et al. 2000. Arterioscler Thromb. Vasc. Biol. 20, 2019.
Pourtau, J., et al. 1998. Blood Coagul Fibrinolysis 9, 609.
Van, D.E., et al. 1995. Blood 85, 3510.