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MABF2061 Anti-TAPA-1 (CD81) Antibody, clone 5A6

Anti-TAPA-1 (CD81), clone 5A6, Cat. No. MABF2061, is a mouse monoclonal antibody that detects TAPA-1 (CD81) and has been tested for use in Flow Cytometry, Inhibition assay, Immunoprecipitation and Western Blotting.

Anti-TAPA-1 (CD81) Antibody, clone 5A6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD81, TAPA1, TSPAN28 UniProt Number: P60033
MABF2061
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, Inhibition, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2061
DescriptionAnti-TAPA-1 (CD81) Antibody, clone 5A6
Alternate Names
  • CD81 antigen
  • 26 kDa cell surface protein TAPA-1
  • Target of the antiproliferative antibody 1
  • Tetraspanin-28
  • Tspan-28
Background InformationCD81 antigen (UniProt: P60033; also known as 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81) is encoded by the CD81 (also known as TAPA1, TSPAN28) gene (Gene ID: 975) in human. TAPA-1 is a nonglycosylated, homodimeric, multi-pass membrane protein of the tetraspanin (TM4SF) family. It is expressed in a wide range of cells, including hematolymphoid, neuroectodermal, and mesenchymal tumor cell lines and is reported to play a role in the regulation of lymphoma cell growth. It contains four transmembrane domains, two extracellular loops, and short amino and carboxyl termini that are intracellular. TAPA-1 is shown to affect cell adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells it is a part of a complex involving CD21, CD19, and Leu13 and this complex is reported to reduce the threshold for B cell activation via the B cell receptor by bridging antigen specific recognition and CD21-mediated complement recognition. On T cells, it is shown to associate with CD4 and CD8 and provides a costimulatory signal with CD3. It is also reported to act as a receptor for hepatitis C virus (HCV) in hepatocytes. Mutations in CD81 gene cause common variable immunodeficiency 6 (CVID6) that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. (Levy S., et al. (1998). Annu. Rev. Immunol.16; 89-109; Oren, R., et al. (1990). Mol. Cell Biol. 10(8); 4007-15).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 containing PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-TAPA-1 (CD81), clone 5A6, Cat. No. MABF2061, is a mouse monoclonal antibody that detects TAPA-1 (CD81) and has been tested for use in Flow Cytometry, Inhibition assay, Immunoprecipitation and Western Blotting.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected TAPA-1 (CD81) in Western Blotting applications (Clark, K.L., et. al. (2004). J Biol Chem. 279(19):19401-6).

Immunoprecipitation Analysis: A representative lot immunoprecipitated TAPA-1 (CD81) in Immunoprecipitation applications (Clark, K.L., et. al. (2004). J Biol Chem. 279(19):19401-6; Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).

Flow Cytometry Analysis: A representative lot detected TAPA-1 (CD81) in Flow Cytometry applications (van Zelm, M.C., et. al. (2010). J Clin Invest. 120(4):1265-74; Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).

Inhibition: A representative lot inhibited the growth and proliferation of OCI-LY8 cell line. (Oren, R., et. al. (1990). Mol Cell Biol. 10(8):4007-15).
Biological Information
ImmunogenOCI-LY8 whole cells (Cellosaurus cell line).
Clone5A6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5A6 detects TAPA-1 (CD81) in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD81
  • TAPA1
  • TSPAN28
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.81 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HUH-7 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected TAPA-1 (CD81) in one million HUH-7 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2061 04054839402203

Documentation

Anti-TAPA-1 (CD81) Antibody, clone 5A6 Certificates of Analysis

TitleLot Number
Anti-TAPA-1 (CD81), clone 5A6 - 3379901 3379901
Anti-TAPA-1 (CD81), clone 5A6 - 3455542 3455542
Anti-TAPA-1 (CD81), clone 5A6 - 3615445 3615445
Anti-TAPA-1 (CD81), clone 5A6 - 3872433 3872433
Anti-TAPA-1 (CD81), clone 5A6 - 3896367 3896367
Anti-TAPA-1 (CD81), clone 5A6 - 3910131 3910131
Anti-TAPA-1 (CD81), clone 5A6 - 3932887 3932887
Anti-TAPA-1 (CD81), clone 5A6 - 3932891 3932891
Anti-TAPA-1 (CD81), clone 5A6 Monoclonal Antibody 3143117
Anti-TAPA-1 (CD81), clone 5A6 Monoclonal Antibody 3792064
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