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MABN2394 Anti-Syntaxin-3B Antibody, clone 12E5

Anti-Syntaxin-3B clone 12E5, Cat. No. MABN2394, is a highly specific mouse monoclonal antibody that targets Syntaxin 3B and has been tested in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Syntaxin-3B Antibody, clone 12E5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Stx3 UniProt Number: Q64704
MABN2394
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, BICC, WB, IHC, IP, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2394
DescriptionAnti-Syntaxin-3B Antibody, clone 12E5
Alternate Names
  • STX3
Background InformationSyntaxin-3 (UniProt: Q64704; also known as STX3) is encoded by the Stx3 gene (Gene ID: 20908) in murine species. Syntaxin-3B is a single-pass type IV membrane protein that is specific for ribbon synapses of the retina. Stx3 gene codes for four different splice forms, Syntaxin 3A, 3B, 3C and 3D. Syntaxin 3A and 3B have an identical N-terminal domain but differ in the C-terminal half of the SNARE domain and the C-terminal transmembrane domain. These two domains are thought to be directly involved in synaptic vesicle fusion. Syntaxin 3 protein is found in all ribbon synapses of the retina. This type of synapse in the retina is formed by rod and cone photoreceptors, as well as by bipolar neurons. Syntaxin 3B has been shown to be important for synaptic vesicle exocytosis in retinal bipolar cells indicating a specific role for Syntaxin 3B in ribbon synapses. Syntaxin 3B can be phosphorylation at Thr14 by CAM Kinase II is shown to increase its binding to SNAP-25. (Ref.: Curtis, LB et al. (2008). J. Comp. Neurol. 510(5): 550-559; Liu, X et al. (2014). Mol. Cell. Neurosci. 60(1): 53-62).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Syntaxin-3B clone 12E5, Cat. No. MABN2394, is a highly specific mouse monoclonal antibody that targets Syntaxin 3B and has been tested in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Syntaxin-3B in human retina and mouse eye tissues.

Western Blotting Analysis: A representative lot detected Syntaxin-3B in Western Blotting applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunoprecipitation Analysis: A representative lot detected Syntaxin-3B in Immunoprecipitation applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunohistochemistry Analysis: A representative lot detected Syntaxin-3B in Immunohistochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunocytochemistry Analysis: A representative lot detected Syntaxin-3B in Immunocytochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).
Biological Information
ImmunogenGST-tagged recombinant murine Syntaxin 3, isoform B that lacks the transmembrane domain.
Clone12E5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12E5 is a highly specific antibody against the B isoform of Syntaxin 3, which is abundantly expressed in photoreceptors of the retina.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Bovine
Species Reactivity NoteBovine, Human, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Stx3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~33 kDa observed; 32.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retinal tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Syntaxin-3B in 10 µg of human retinal tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2394 04054839197741