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04-1549 Anti-SAP130 Antibody, clone 1YO 1B10

Use Anti-SAP130 Antibody, clone 1YO 1B10 (Mouse Monoclonal Antibody) validated in WB, ICC to detect SAP130 also known as 130 kDa Sin3-associated polypeptide, SF3b130.

Anti-SAP130 Antibody, clone 1YO 1B10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, Chromatin Biology Gene Symbol: SAP130, Sf3b3, Kiaa0017 UniProt Number: Q921M3
04-1549
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCMAscitesMonoclonal Antibody
Description
Catalogue Number04-1549
DescriptionAnti-SAP130 Antibody, clone 1YO 1B10
Alternate Names
  • 130 kDa Sin3-associated polypeptide
  • Sin3-associated polypeptide p130
  • Sin3A-associated protein, 130kDa
  • histone deacetylase complex subunit SAP130
  • mSin3A-associated protein 130
    Spliceosome-associated protein 130
  • Pre-mRNA-splicing factor SF3b 130 kDa subunit
  • SF3b130
Background InformationSAP130, also known as Splicing factor 3B subunit 3, is part of the RNA splicing machinery. It is expressed by the Sf3b3 gene and encodes subunit 3 of the splicing factor 3b protein complex. As a component of this protein complex, it is associated with U2 snRNP and recruited to prespliceosomal complexes. Splicing factor 3b along with splicing factor 3a and a 12S RNA, form the U2 small nuclear ribonucleoprotein complex (U2 snRNP). In pre-mRNA’s, the splicing factor 3b/3a complex binds upstream of intron branch sites possibly anchoring the U2 snRNP to the pre-mRNA.
SAP130 is also a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes are believed to function in chromatin modification, transcription, splicing, and DNA repair.
References
Product Information
FormatAscites
Control
  • RAW264.7 cell lysate
PresentationUnpurified mouse monoclonal IgG1κ preservative-free ascites.
Quality LevelMQ100
Applications
ApplicationUse Anti-SAP130 Antibody, clone 1YO 1B10 (Mouse Monoclonal Antibody) validated in WB, ICC to detect SAP130 also known as 130 kDa Sin3-associated polypeptide, SF3b130.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected SAP130 in NIH/3T3 cells.
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to mouse SAP130.
EpitopeUnknown
Cloneclone 1YO 1B10
HostMouse
SpecificityThis antibody recognizes SAP130.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SAP130
  • Sf3b3
  • Kiaa0017
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron by similarity.

SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of splicing factor SF3B which is composed of at least eight subunits; SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interaction between SF3B3 and SF3B1 is tighter than the interaction between SF3B3 and SF3B2. Associates with the STAGA transcription coactivator-HAT complex. Interacts with SUPT3H by similarity.

SUBCELLULAR LOCATION: Nucleus by similarity.

SEQUENCE SIMILARITIES: Belongs to the RSE1 family.
Molecular Weight ~ 136 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected SAP130 on 10 µg of RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1549 04053252510854