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ABE999 Anti-RPB3

ABE999
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE999
DescriptionAnti-RPB3
Alternate Names
  • RPB3
  • DNA-directed RNA polymerase II subunit RPB3
  • RNA polymerase II subunit 3
  • RNA polymerase II subunit B3
  • DNA-directed RNA polymerase II 33 kDa polypeptide
  • DNA-directed RNA polymerase II subunit C
  • RPB31
Background InformationRPB3 is one of 12 subunits that make up the complete RNA polymerase, Pol II, the primary RNA polymerase in cellular metabolism. RPB3 is part of the core element of Pol II complex. RPB3 has a central large cleft and the clamp element that moves to open and close the cleft during RNA synthesis. RPB3 naturally localizes to the nucleus and expressed in all cell types and tissues. RPB3 also plays critical roles in RNA splicing and capping, key items of RNA function.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RPB3 Antibody is an antibody against RPB3 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human RPB3 near the Near C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Rat, Mouse. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2C
  • A-152E5.7
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~35 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RPB3 in 10 µg of HUVEC cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE999 04055977135350

Documentation

Anti-RPB3 SDS

Title

Safety Data Sheet (SDS) 

Anti-RPB3 Certificates of Analysis

TitleLot Number
Anti-RPB3 - 3173786 3173786
Anti-RPB3 - 3735352 3735352
Anti-RPB3 - 3764357 3764357
Anti-RPB3 - 3982598 3982598
Anti-RPB3 - 4062249 4062249
Anti-RPB3 -Q2550840 Q2550840
Anti-RPB3 Polyclonal Antibody - 3932875 3932875

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.