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AB5860-200UL Anti-Potassium Channel TREK-1 Antibody

Detect Potassium Channel TREK-1 using this Anti-Potassium Channel TREK-1 Antibody validated for use in WB.

Anti-Potassium Channel TREK-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNK2, hTREK-1e, MGC126742, hTREK-1c, TREK, TPKC1, MGC126744, TREK1, TREK-1, K2p2.1 UniProt Number: O95069
AB5860-200UL
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5860-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Potassium Channel TREK-1 Antibody
Alternate Names
  • Twik-Related K+ Channel 1
  • Kcnk2
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationLyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min.)
Quality LevelMQ100
Applications
ApplicationDetect Potassium Channel TREK-1 using this Anti-Potassium Channel TREK-1 Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide corresponding amino acids 8-28 of human TREK-1 (Accession numbers AAF89743, AAD47569).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes TREK-1 (Twik-Related K+ Channel 1, Kcnk2). The epitope does not share homology with any other known proteins.
Species Reactivity
  • Human
  • Rat
Species Reactivity Note It is expected that the antibody will also react with mouse due to sequence homology (17/18). Other species have not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • KCNK2
  • hTREK-1e
  • MGC126742
  • hTREK-1c
  • TREK
  • TPKC1
  • MGC126744
  • TREK1
  • TREK-1
  • K2p2.1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O95069 # Outward rectifying potassium channel.
SIZE: 426 amino acids; 47093 Da
SUBUNIT: Homodimer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: SwissProt: O95069 ## Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
MISCELLANEOUS: Activated by volatile general anesthetics such as chloroform, halothane and isoflurane.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Global Trade Item Number
Catalogue Number GTIN
AB5860-200UL 04053252323867