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ABE1863 Anti-PAGR1 (PA1)

Anti-PAGR1 (PA1), Cat. No. ABE1863, is a highly specific rabbit polyclonal antibody that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.

ABE1863
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE1863
DescriptionAnti-PAGR1 (PA1)
Alternate Names
  • PAXIP1-associated glutamate-rich protein 1
  • Glutamate-rich co-activator interacting with SRC11
  • GAS1
  • PAXIP1-associated protein 1
  • PTIP-associated protein 1
Background InformationPAXIP1-associated glutamate-rich protein 1 (UniProt: Q9BTK6; also known as Glutamate-rich co-activator interacting with SRC1, GAS, PAXIP1-associated protein 1, PTIP-associated protein 1) is encoded by the PAGR1 (also known as C16orf53, PA1) gene (Gene ID: 79447) in human. It associates with the histone methyltransferase MLL3/4 complex, which suggests that it plays a role in epigenetic and transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL3/4 complex. It is proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL3/4 complex. During immunoglobulin class switching in activated B-cells, it is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL3/4 complex. PAGR1 protein is reported to act as a transcriptional cofactor for estrogen receptor. It interacts with GR (NR3C1) and inhibits the GR transactivation.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PAGR1 (PA1), Cat. No. ABE1863, is a highly specific rabbit polyclonal antibody that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: A representative lot detected PAGR1 (PA1) in Western Blotting applications (Lee, J.E., et. al. (2013). Elife. 2:e01503; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEF's (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
Biological Information
ImmunogenHis-tagged full-length human recombinant PAXIP1-associated protein 1.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects PAXIP1-associated glutamate-rich protein 1 in human and mouse.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PAGR1
  • C16orf53
  • PA1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~42 kDa observed; 27.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1863 04054839233159

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