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MABN2257 Anti-Nudel (NDEL1) Antibody, clone 15D5.1

Anti-Nudel (NDEL1), clone 15D5.1, Cat. No. MABN2257, is a highly specific mouse monoclonal antibody that targets Nuclear distribution protein nudE-like 1 (Nudel) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Nudel (NDEL1) Antibody, clone 15D5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NDEL1, MITAP1, NUDEL UniProt Number: Q9GZM8
MABN2257
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2257
DescriptionAnti-Nudel (NDEL1) Antibody, clone 15D5.1
Alternate Names
  • Nuclear distribution protein nudE-like 1
  • Protein Nudel
  • Mitosin-associated protein 1
Background InformationNuclear distribution protein nudE-like 1 (UniProt: Q9GZM8; also known as Protein Nudel, Mitosin-associated protein 1) is encoded by the NDEL1 (also known as EOPA, MITAP1, NUDEL) gene (Gene ID: 81565) in human. Nudel protein belongs to the nude family and is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. Its expression is shown to peak during mitosis. Nudel is undergoes phosphorylation during mitosis. Three isoforms of Nudel have been reported that are produced by alternative splicing. Nudel is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. It may regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome and is shown to positively regulate the activity of the minus-end directed microtubule motor protein dynein. It enhances dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends and is shown to be essential for several dynein- and microtubule-dependent processes, such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Nudel is required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate and also plays a role in the regulation of neurite outgrowth. It localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nudel (NDEL1), clone 15D5.1, Cat. No. MABN2257, is a highly specific mouse monoclonal antibody that targets Nuclear distribution protein nudE-like 1 (Nudel) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nuclear distribution protein nudE-like 1 (Nudel) in human colon, human cerebral cortex, and human tonsil tissues.
Biological Information
ImmunogenGST/His-taged recombinant fragment corresponding to 101 amino acids from the N-terminal region of human Nuclear distribution protein nudE-like 1 (Nudel).
Clone15D5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 15D5.1 detects Nuclear distribution protein nudE-like 1 (Nudel) in human and murine cells. It targets an epitope within 101 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NDEL1
  • MITAP1
  • NUDEL
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40 kDa observed; 38.38 kda calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nuclear distribution protein nudE-like 1 (Nudel) in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2257 04054839402050