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AB1526SP Anti-Nerve Growth Factor-β Antibody

AB1526SP
500 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, H, M, RELISA, WB, IHC, FUNCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB1526SP
Replaces04-1119
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nerve Growth Factor-β Antibody
Alternate Names
  • NGFbeta
Product Information
FormatPurified
PresentationPurified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Quality LevelMQ100
Applications
ApplicationAnti-Nerve Growth Factor-β Antibody detects level of Nerve Growth Factor-β & has been published & validated for use in ELISA, WB, IH.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry
  • Affects Function
Application NotesImmunohistochemistry: 1-2 μg/mL (see suggested protocol).

Immunoblotting: 1-2 μg/mL

Inhibition of biological activity: 1-2 μg/mL
BIOLOGICAL ACTIVITY: This antibody will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro.

ELISA: 1-2 μg/mL

Use neat for in vivo animal studies.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenMouse nerve growth factor-beta purified from salivary glands.
HostRabbit
SpecificityNerve growth factor-beta (NGF). Less than 1% cross-reactivity with recombinant human NT3, NT4 and BDNF by ELISA.
Species Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Gene Symbol
  • NGFB
  • MGC161426
  • HSAN5
  • NGF
  • Beta-NGF
  • MGC161428
Non-Reactive Species
  • Bovine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01138 # Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
SIZE: 241 amino acids; 26959 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
DISEASE: SwissProt: P01138 # Defects in NGFB are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. Hereditary sensory and autonomic neuropathies are characterized by loss of pain sensation in combination with other sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self- mutilation. The autonomic involvement is variable.
SIMILARITY: SwissProt: P01138 ## Belongs to the NGF-beta family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to one year after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) may be added for additional stability. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size500 µg
Global Trade Item Number
Catalogue Number GTIN
AB1526SP 04053252581045