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05-369X-555 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555

Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: ATP1A3, MGC13276, RDP, IIIsubunit)., DYT12, +K, Na UniProt Number: Q9N0Z6
05-369X-555
25 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, M, Mk, Po, R, Rb, Sh, XnFC, IF, WBMAlexaFluor®555Monoclonal Antibody
Description
Catalogue Number05-369X-555
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555
References
Product Information
FormatAlexaFluor®555
Control
  • Included Negative Control: Catalog # 16-239, Alexa Fluor® 555-conjugated Normal Mouse IgG.
Presentation25µg Alexa Fluor® 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide. Liquid at 4°C.
Quality LevelMQ100
Applications
ApplicationDetect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Western Blotting
Biological Information
ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
CloneC464.6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecific for the α-1 subunit isoform.
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Monkey
  • Pig
  • Rat
  • Rabbit
  • Sheep
  • Xenopus
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
Gene Symbol
  • ATP1A3
  • MGC13276
  • RDP
  • IIIsubunit).
  • DYT12
  • +K
  • Na
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
SIZE: 1013 amino acids; 111749 Da
SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
Molecular Weight113 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40μg of rat brain microsomal preparation.

Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.

Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsDo Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-369X-555 04053252277658

Documentation

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555 SDS

Title

Safety Data Sheet (SDS) 

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555 Certificates of Analysis

TitleLot Number
Anti-Na+/K+ ATPase #945;-1, clone C464.6, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1#954;) - 2113340 2113340
Anti-Na+/K+ ATPase #945;-1, clone C464.6, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1#954;) - 2121956 2121956
Anti-Na+/K+ ATPase #945;-1, clone C464.6, Alexa Fluor#174; 555 conjugate - 2056623 2056623
Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1) - DAM1408966 DAM1408966
Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor#174; 555 conjugate - 2036871 2036871
Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor#174; 555 conjugate - 2045873 2045873
Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) - 2322509 2322509
Anti-Na+/K+ ATPase alpha;-1, clone C464.6, Alexa Fluorreg; 555 conjugate - D6D031 D6D031
Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 2455747
Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1κ) 3012214
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Catalogue Number Description
16-243 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488

Alternative Format

Catalogue Number Description
05-369 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6
16-243 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies