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ABT1488 Anti-MCAM/CD146 Antibody

Anti-MCAM/CD146 Antibody is an antibody against MCAM/CD146 for use in Western Blotting, Immunoprecipitation, Immunohistochemistry.

Anti-MCAM/CD146 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: MCAM, MUC18 UniProt Number: P43121
ABT1488
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT1488
Replaces04-1147
DescriptionAnti-MCAM/CD146 Antibody
Alternate Names
  • Cell surface glycoprotein MUC18
  • CD146
  • Cell surface glycoprotein P1H12
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • Melanoma cell adhesion molecule
  • S-endo 1 endothelial-associated antigen
Background InformationCell surface glycoprotein MUC18 (UniProt P43121; also known as CD146, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen) is encoded by the MCAM (also known as MUC18) gene (Gene ID 4162) in human. MUC18/CD146 is an 113 kDa transmembrane immunoglobulin superfamily glycoprotein mainly expressed on endothelial cells and smooth muscle cells where it functions as a cell-to-cell adhesion molecule, while little or no expression is found among epithelial cells and leukocytes (e.g. macrophages and neutrophils). Originally characterized as a protein overexpressed in malignant melanoma cells and adopted the name MCAM (melanoma cell adhesion molecule), MUC18/CD146 up-regulation is also reported in airway epithelial cells of patients with asthma and chronic obstructive pulmonary disease (COPD). Cellular overexpression and knockdown studies indicate that MUC18/CD146 plays a pro-inflammatory role in response to stimulation with various TLR agonists. MUC18/CD146 is produced with a signal peptide (a.a. 1-23) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 24-559), followed by a transmembrane domain (a.a. 560-583) and a cytoplasmic domain (a.a. 584-646). The extracellular region contains two V-type (a.a. 24-129 and 139-242) and three C2-type (a.a. 244-330, 335-424, 430-510) Ig-like domains.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MCAM/CD146 Antibody is an antibody against MCAM/CD146 for use in Western Blotting, Immunoprecipitation, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
Application NotesImmunoprecipitation Analysis: 10 ug from a representative lot immunoprecipitated MCAM/CD146 from 500 µg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MCAM/CD146 in human brain, kidney, and melanoma tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the third C2-type Ig-like domain of human MCAM/CD146.
EpitopeC2-type Ig-like domain 3.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with both spliced isoforms of human MCAM/CD146 reported by UniProt (P43121).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MCAM
  • MUC18
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~113 kDa observed. Target band size appears larger than the calculated molecular weights of 70.90 kDa (isoform 1) and 56.89 kDa (isoform 2) due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected MCAM/CD146 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT1488 04055977332261