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05-1066-25UG Anti-Insulin Antibody, clone E11D7 (mouse monoclonal)

Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Metabolism Research Sub-Category: Insulin/Energy Signaling, Glucose/Glycogen Metabolism, Metabolic Hormones & Receptors Gene Symbol: INS, proinsulin, insulin UniProt Number: P01308
05-1066-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue Number05-1066-25UG
DescriptionAnti-Insulin Antibody, clone E11D7 (mouse monoclonal)
Background InformationInsulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
References
Product Information
FormatPurified
PresentationPurified mouse monolconal IgG1 in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
Quality LevelMQ100
Applications
ApplicationAnti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
Application NotesELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Biological Information
ImmunogenHuman recombinant Insulin
EpitopeInsulin
CloneE11D7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes insulin
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol
  • INS
  • proinsulin
  • insulin
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Molecular Weight6 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1066-25UG 04054839337710

Documentation

Anti-Insulin Antibody, clone E11D7 (mouse monoclonal) Certificates of Analysis

TitleLot Number
Anti-Insulin, clone E11D7 (mouse monoclonal) - 3792009 3792009
Anti-Insulin, clone E11D7 (mouse monoclonal) - 4059350 4059350
Anti-Insulin, clone E11D7 (mouse monoclonal) - 4192590 4192590
Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3090199
Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3035062
Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3059617
Anti-Insulin, clone E11D7 - 3389002 3389002