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GF39 Anti-HGF (Scatter Factor) (Ab-1) Mouse mAb (4C12.1)

Anti-HGF (Scatter Factor) (Ab-1), mouse monoclonal, clone 4C12.1, recognizes the ~82 kDa hepatocyte growth factor in macrophages, endothelial & Kupffer cells. It is validated for use in WB & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Scatter Factor, Anti-Hepatocyte Growth Factor, Anti-SF, Anti-Hepatopoeitin A

GF39
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GF39-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~82 kDa hepatocyte growth factor protein in macrophages, endothelial, and Kupffer cells.
Catalogue NumberGF39
Brand Family Calbiochem®
SynonymsAnti-Scatter Factor, Anti-Hepatocyte Growth Factor, Anti-SF, Anti-Hepatopoeitin A
References
ReferencesToi, M., et al. 1998. Clin. Cancer Res. 4, 659.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp. 212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp. 212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol. 12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp. 212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp. 212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp. 212, 119.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlEndothelial cells, Kupffer cells, or macrophages
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human HGF protein
ImmunogenHuman
Clone4C12.1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF39-100UG 04055977220131

Documentation

Anti-HGF (Scatter Factor) (Ab-1) Mouse mAb (4C12.1) SDS

Title

Safety Data Sheet (SDS) 

Anti-HGF (Scatter Factor) (Ab-1) Mouse mAb (4C12.1) Certificates of Analysis

TitleLot Number
GF39

References

Reference overview
Toi, M., et al. 1998. Clin. Cancer Res. 4, 659.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp. 212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp. 212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol. 12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp. 212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp. 212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp. 212, 119.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2007 RFH
SynonymsAnti-Scatter Factor, Anti-Hepatocyte Growth Factor, Anti-SF, Anti-Hepatopoeitin A
ApplicationImmunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~82 kDa HGF protein.
BackgroundHepatocyte Growth Factor (HGF), also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotrophic growth factor which is produced by mesodermally derived cells such as endothelial cells, Kupffer cells/macrophages, and hepatic fat-storing cells. HGF/SF is a potent effector of growth, movement, differentiation and invasiveness of epithelial and cancer cells. It can induce epithelial-mesenchymal conversion of epithelial cells in culture, support epithelial branching duct formation in the developing lung and elicit a potent organotrophic function for regeneration of liver, kidney and lung through epithelial-stromal interactions. HGF/SF is also a potent angiogenic molecule whose activity such as cell migration, proliferation, protease production, invasion and capillary tube formation is mediated through direct actions on vascular endothelial cells. Recent evidence has shown that HGF/SF is overexpressed in invasive human cancer, relative to non-invasive and benign conditions. In invasive breast cancers, the expression of HGF/SF is strongly correlated with von Willebrand Factor, a marker of endothelial cells. The invasive and metastatic signals given by HGF/SF are transduced through the c-met proto-oncogene product, a transmembrane tyrosine kinase that functions as the HGF/SF receptor.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human HGF protein
Clone4C12.1
IsotypeIgG2b
Specieshuman
Positive controlEndothelial cells, Kupffer cells, or macrophages
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Standard Handling
ReferencesToi, M., et al. 1998. Clin. Cancer Res. 4, 659.
Bardelli, A. and Comoglio, P.M. 1997. Ciba Found. Symp. 212, 133.
Birchmeier, C., et al. 1997. Ciba Found. Symp. 212, 16.
Jiang, W.G. and Hiscox, S. 1997. Histol. Histopathol. 12, 537.
Matsumoto, K. and Nakamura, T. 1997. Ciba Found. Symp. 212, 198.
Rosen, E.M., et al. 1997. Ciba Found. Symp. 212, 215.
Vande Woude, G.F., et al. 1997. Ciba Found Symp. 212, 119.