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ABC955 Anti-GADD153/DDIT3/CHOP Antibody

This Anti-GADD153/DDIT3/CHOP antibody is validated for use in Western Blotting for the detection of GADD153/DDIT3/CHOP.

Anti-GADD153/DDIT3/CHOP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DDIT3, CHOP, CHOP10, GADD153 UniProt Number: P35638
ABC955
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC955
DescriptionAnti-GADD153/DDIT3/CHOP Antibody
Alternate Names
  • DNA damage-inducible transcript 3 protein
  • DDIT-3
  • C/EBP zeta
  • C/EBP-homologous protein
  • CHOP
  • C/EBP-homologous protein 10
  • CHOP-10
  • CCAAT/enhancer-binding protein homologous protein
  • Growth arrest and DNA damage-inducible protein GADD153
  • GADD153/DDIT3/CHOP
Background InformationDNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GADD153/DDIT3/CHOP antibody is validated for use in Western Blotting for the detection of GADD153/DDIT3/CHOP.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human GADD153/DDIT3/CHOP near the N-terminus.
EpitopeNear N-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Rat, Monkey, Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DDIT3
  • CHOP
  • CHOP10
  • GADD153
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~29 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Tunicamycin treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD153/DDIT3/CHOP in 10 µg of Tunicamycin treated HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC955 04055977116991

Documentation

Anti-GADD153/DDIT3/CHOP Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-GADD153/DDIT3/CHOP Antibody Certificates of Analysis

TitleLot Number
Anti-GADD153/DDIT3/CHOP Q2485583
Anti-GADD153/DDIT3/CHOP - 3188636 3188636
Anti-GADD153/DDIT3/CHOP - 3324192 3324192
Anti-GADD153/DDIT3/CHOP - 3749979 3749979
Anti-GADD153/DDIT3/CHOP - 3919640 3919640
Anti-GADD153/DDIT3/CHOP - 4125697 4125697
Anti-GADD153/DDIT3/CHOP -2730018 2730018
Anti-GADD153/DDIT3/CHOP -2789067 2789067
Anti-GADD153/DDIT3/CHOP Polyclonal Antibody 2937367
Anti-GADD153/DDIT3/CHOP Polyclonal Antibody 2953852