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MABN875 Sigma-AldrichAnti- GABAA Antibody γ2 Subunit, clone KC4-8A7

This Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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References
Technical Information

Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: GABRG2 UniProt Number: P18507

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, B	WB, IH(P), IP, ELISA	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN875
Description	Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7
Alternate Names	Gamma-aminobutyric acid receptor subunit gamma-2 GABA(A) receptor subunit gamma-2 GABAA γ2 Subunit
Background Information	Gamma-aminobutyric acid (GABA) receptor subunit gamma-2 (UniProt P18507; also known as GABA(A) receptor subunit gamma-2) is encoded by the GABRG2 (also known as CAE2, ECA2, GEFSP3) gene (Gene ID 2566) in human. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Upon GABA binding and activation, the GABAA receptor selectively conducts chloride ions through its pore, resulting in hyperpolarization of the neuron. The GABAAR is a pentameric complex composed of two alpha, two beta, and one gamma subunit arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The gamma 2 subunit is initially produced with an signal peptide sequence (a.a. 1-39), which is then cleaved off to yield the mature subunit (a.a. 40 – 467).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Immunoprecipitation ELISA
Application Notes	Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of mouse brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABAA γ2 Subunit in human cerebral cortex tissue. Western Blotting Analysis: A representative lot detected human GABAA gamma2 subunit cytoplasmic domain GST fusion protein, as well as endogenous GABAA gamma2 subunit in purified bovine brain GABAA receptor complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311). Immunoprecipitation Analysis: A representative lot immunoprecipitated GABAA receptor complex from rat cerebral cortex membrane extracts, as well as purified bovine brain GABAA receptor complex as measured by ligand-binding activity associated with the immune complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311). ELISA Analysis: A representative lot detected human GABAA gamma2 cytoplasmic domain GST fusion protein, but not GST (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the cytoplasmic domain of human GABAA γ2 Subunit.
Epitope	cytoplasmic domain
Clone	KC4-8A7
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Expected to react with all three spliced isoforms of GABA(A) receptor gamma 2 subunit (GABRG2), but not gamma 1 (GABRG1) or gamma 3 (GABRG3) subunit.
Isotype	IgG2aκ
Species Reactivity	Human Rat Mouse Bovine
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_944494
Gene Symbol	GABRG2
Purification Method	Protein G Purified
UniProt Number	P18507
Molecular Weight	~45 kDa observed. Molecular weight bands were observed at ~45 kDa for both rat and mouse brain tissue lysates. However, for human brain tissue lysates, we detected a molecular weight at ~58 kDa, as predicted by Uniprot.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected GABAA γ2 Subunit in 10 µg of rat brain tissue lysate. Note: For Western blotting analysis, DO NOT BOIL samples prior to electrophoresis. After dissociating receptor complex with SDS sample buffer, remove non-solublized material by centrifugation.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABN875	04055977277371

Documentation

Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 SDS

Title
Safety Data Sheet (SDS)

Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 Certificates of Analysis

Title	Lot Number
Anti- GABAA γ2 Subunit, -Q2602138	Q2602138
Anti- GABAA γ2 Subunit, clone KC4-8A7 - 3310101	3310101

References

Reference overview	Pub Med ID
Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors. Fernando, LP; Khan, ZU; McKernan, RM; De Blas, AL Journal of neurochemistry 64 1305-11 1995 Show Abstract The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.	7861163

Reference overview

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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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