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DR1061 Anti-ENO1 Mouse mAb (8G8)

Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).

Anti-ENO1 Mouse mAb (8G8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-α Enolase 1

DR1061
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1061-100UG 100 μg
Description
OverviewRecognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
Catalogue NumberDR1061
Brand Family Calbiochem®
SynonymsAnti-α Enolase 1
Application Data
Detection of human ENO1 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1-5 µg/ml). Detection: chmiluminescence.

Detection of human ENO1 by Immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (10 µg/ml). Detection: fluorescence.

Detection of human ENO1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lymphoma tissue. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1 µg/ml). Detection: colorimetric (DAB).
References
ReferencesHo, J.A., et al. 2010. Anal. Chem. 82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.
Product Information
FormLiquid
FormulationIn PBS, pH 7.30.
Positive controlMCF-7 cells, Human lymphoma tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (1 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
Clone8G8
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1061-100UG 04055977226027

Documentation

Anti-ENO1 Mouse mAb (8G8) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Ho, J.A., et al. 2010. Anal. Chem. 82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-α Enolase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (1 µg/ml)
Application Data
Detection of human ENO1 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1-5 µg/ml). Detection: chmiluminescence.

Detection of human ENO1 by Immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (10 µg/ml). Detection: fluorescence.

Detection of human ENO1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lymphoma tissue. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.
BackgroundThis protein is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
HostMouse
ImmunogenFull-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
Clone8G8
IsotypeIgG₁
Specieshuman
Positive controlMCF-7 cells, Human lymphoma tissue, HeLa cells
FormLiquid
FormulationIn PBS, pH 7.30.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesHo, J.A., et al. 2010. Anal. Chem. 82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.