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MABE1011 Anti-DNA Ligase 3 Antibody, clone 7H6.1

This Anti-DNA Ligase 3 Antibody, clone 7H6.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of DNA Ligase 3.

Anti-DNA Ligase 3 Antibody, clone 7H6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: LIG3 UniProt Number: P49916
MABE1011
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1011
DescriptionAnti-DNA Ligase 3 Antibody, clone 7H6.1
Alternate Names
  • DNA ligase 3
  • DNA Ligase 3
  • DNA ligase III
  • Polydeoxyribonucleotide synthase [ATP] 3
Background InformationDNA ligase 3 (EC 6.5.1.1; UniProt P49916; also known as DNA ligase III, ATP-dependent polydeoxyribonucleotide synthase [ATP] 3) is encoded by the LIG3 gene (Gene ID 3980) in human. Three genes (LIG1, LIG3, and LIG4) in human genome encode ATP-dependent DNA ligases required for repairing DNA single- and double-strand breaks (SSB and DSB) and completing repair events initiated by the excision of mismatched and damaged bases. Among the human LIG genes, the LIG3 is unique in that it encodes multiple isforms with different N- and C-termini via alternative translation initiation and splicings. The "breast cancer susceptibility protein 1 C-terminal" (BRCT) domain present at the C-terminal end of the canonical isoform 1 (a.a. 933-1009) and isoform 3 is replaced by a short positively charged sequence that acts as a nuclear localization signal in spliced isoform 2 and isoform 4 (also known as the beta isoform). In addition, the N-terminal end of isoform 3 (also known as the alpha isoform) and isoform 4 do not contain the 87 a.a. mitochondrial targeting sequence present in isoform 1 and isoform 2. The LIG3 is the only gene encodes mitochondria DNA ligase (i.e. isoforms 1 & 2), inactivation of the LIG3 gene results in loss of mitochondrial function and cell death. Isoforms 1 and 3 interact with the scaffold protein XRCC1 via their BRCT domain, the XRCC1 in turn recruits a large number of proteins involved in base excision and single-strand break repair.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-DNA Ligase 3 Antibody, clone 7H6.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of DNA Ligase 3.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected DNA Ligase 3 in HeLa, A431, HUVEC and NIH/3T3 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal of human DNA Ligase 3.
EpitopeC-terminal
Clone7H6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all four spliced isoforms.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LIG3
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~113/106 kDa observed. 112.9 kDa (isoform 1), 106.0 kDa (isoform 2), 102.7 kDa (isoform 3), 95.8 kDa (isoform 4) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA Ligase 3 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1011 04055977297430