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MAB030 Anti-DNA Antibody, double stranded, clone BV16-13

MAB030
500 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AELISA, RIA, ICCMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMAB030
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-DNA Antibody, double stranded, clone BV16-13
Product Information
FormatCulture Supernatant
PresentationUnpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Quality LevelMQ100
Applications
ApplicationDetect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
Key Applications
  • ELISA
  • Radioimmunoassay
  • Immunocytochemistry
Application NotesEIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Biological Information
Epitopedouble stranded, 28% reactive with single stranded
CloneBV16-13
HostMouse
SpecificityDouble stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
IsotypeIgG2aκ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size500 µL
Global Trade Item Number
Catalogue Number GTIN
MAB030 04053252610851