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MAB3406-40UG Anti-Cytokeratin Pan Antibody, clone Lu5

This Anti-Cytokeratin Pan Antibody, clone Lu5 is validated for use in IH for the detection of Cytokeratin Pan.

Anti-Cytokeratin Pan Antibody, clone Lu5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytokeratins
MAB3406-40UG
40 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MaIHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3406-40UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytokeratin Pan Antibody, clone Lu5
References
Product Information
FormatPurified
PresentationLiquid. Buffer = 0.02 M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cytokeratin Pan Antibody, clone Lu5 is validated for use in IH for the detection of Cytokeratin Pan.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry: 10-20 μg/mL Optimal working dilutions must be determined by end user.

Tumor Characterization

For tumor characterization a two-step experimental assay is recommended:

1. Characterization of the tissue origin of a tumor with the aid of anti-cytokeratin pan, anti-desmin, anti-glial fibrillary acidic protein, anti-neurofilament and anti-vimentin.

2. Characterization of an epithelial tumor using antibodies to cytokeratin classes which are characteristic for different tissues, e.g. anti-cytokeratin No. 7, anti-cytokeratin No. 18. Sample material: Normal tissue, tumor tissue following surgery or up to 24 h after autopsy, frozen sections or formaldehyde-fixed paraffin embedded sections,

Detection method

Immunohistochemical analysis (detection of the primary antibody e. g. by anti-mouse Ig-peroxidase or anti-mouse Ig-FITC).

Procedure:

Ideal frozen sections are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -15 to -25°C. Excess acetone is allowed to evaporate at 15-25°C. Material fixed in alcohol can also be used.

Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should, however, not be air dried, the excess acetone is removed by briefly washing in phosphate-buffered saline (PBS). Further treatment as follows:

• Overlay the preparation with 10 - 20 μL antibody solution and incubate in a humid chamber for 30 - 60 min at 15-25°C.

• Dip the slide briefly in PBS and then wash in PBS 3 times for 3 min each (using a fresh PBS bath in each case).

• Wipe the margins of the preparation dry, overlay the preparation with 10 - 20 μL of a solution of anti-mouse lg-FITC or anti-mouse Ig-peroxidase and allow to incubate in a humid chamber for 30 min at 15-25°C.

• Wash the slide in PBS as described above.

The preparation must not be allowed to dry out during any of the steps. lf using an indirect immunofluorescence technique, the preparation should be overlaid with a suitable embedding medium (e. g. Mowiol, Hoechst) and examined under the fluorescence microscope. lf a POD-conjugate is used as the secondary antibody, the preparation should be overlaid with a substrate solution (see below) and incubated at 15-25°C until a clearly visible redbrown color develops. A negative control (e.g. only the labeled secondary antibody) should remain unchanged in color during this incubation period.

Subsequently, the substrate is washed off with PBS and the preparation is stained with hemalum stain for about 1 min. The hemalum solution is washed off with PBS, the preparation is embedded and examined.

Substrate solutions:

Aminoethyl-carbazole: Dissolve 2 mg 3-amino-9-ethylcarbazole with 1.2 mL dimethylsulfoxide and add 28.8 mL Tris-HCI, 0.05 M; pH 7.3; and 20 μL 3% H 2 O 2 , (w/v). Prepare solution freshly each day. Diaminobenzidine: Dissolve 25 mg 3, 3'-diaminobenzidine with 50 mL Tris-HCI, 0.05 M; pH 7.3; and add 40 μL 3% H2O2 , (w/v). Prepare solution freshly each day. Note The antibody solutions have to be absolutely free of precipitate! lf necessary, centrifuge the solutions at high speed prior to use.
Biological Information
ImmunogenLung tumor cell lines A549 and A2182.
CloneLu5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody reacts with an epitope which is common to all cytokeratins respectively with a cytokeratin-associated protein. The antigen recognized by anti-cytokeratin pan is present in all types of epithelia. The antibody reacts with epithelial and mesothelial cells. Exception: Secretory cells are not stained and acinus cells, for example, show only weak staining. The antibody does not react with connective tissue, blood or lymphatic vessels, smooth or skeletal muscles. Exception: When higher antibody concentrations are used, weakly positive reactions can be observed with the smooth muscles of the myometrium, the prostata as well as the gastric muscularis mucosa.The antibodies can be used for differential diagnosis of epithelial tumors, distinction from mesenchymal, neural tumors as well as large-cell lymphomas. Anti-cytokeratin pan stains all epithelial tumors (primary tumors as well as metastases). Exception: Only partially positive staining is observed with theca cell tumors, granulosa cell tumors, adrenal cortex tumors. In mixed tumors (e.g. carcinosarkoma, thymoma, adenolymphoma) only the epithelial regions are stained. Tumors of nonepithelial origin do not react with the antibody. Exception: With high antibody concentrations a weak staining of leiomyosarcoma of the uterus is observed.
IsotypeIgG1
Species Reactivity
  • Mammals
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized antibody is stabe when stored at 2-8°C.The reconstituted antibody solution is stable at 2-8°C for three monthes. The solution can be stored in aliquots at -15 to -25 °C for 12 monthes. Avoid repeat freeze/thaw cycles.
Packaging Information
Material Size40 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3406-40UG 04053252725043

Documentation

Anti-Cytokeratin Pan Antibody, clone Lu5 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cytokeratin Pan Antibody, clone Lu5 Certificates of Analysis

TitleLot Number
MOUSE ANTI-CYTOKERATIN PAN - 2540017 2540017
MOUSE ANTI-CYTOKERATIN PAN - 3506475 3506475
MOUSE ANTI-CYTOKERATIN PAN - 3509163 3509163
MOUSE ANTI-CYTOKERATIN PAN - 3711529 3711529
MOUSE ANTI-CYTOKERATIN PAN MONOCLONAL ANTIBODY 3057060
MOUSE ANTI-CYTOKERATIN PAN MONOCLONAL ANTIBODY 2877779
MOUSE ANTI-CYTOKERATIN PAN MONOCLONAL ANTIBODY - 2341904 2341904
MOUSE ANTI-CYTOKERATIN PAN MONOCLONAL ANTIBODY -2587266 2587266

References

Reference overviewPub Med ID
Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5).
von Overbeck, J, et al.
Virchows Archiv. A, Pathological anatomy and histopathology, 407: 1-12 (1985)  1985

Show Abstract 		2409666 2409666

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