Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

OP95 Anti-BRCA2 (Ab-1) Mouse mAb (2B)

Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.

Anti-BRCA2 (Ab-1) Mouse mAb (2B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Breast Cancer Susceptibility Protein

OP95
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP95-100UG Glass bottle 100 μg
Add To Favorites
Description
OverviewRecognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Catalogue NumberOP95
Brand Family Calbiochem®
SynonymsAnti-Breast Cancer Susceptibility Protein
References
ReferencesAndres, J.L., et al. 1998. Oncogene 16, 2229.
Chen, J., et al. 1998. Mol. Cell 2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 5287.
Marmorstein, L.Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13869.
Connor, F., et al. 1997. Nat. Genet. 17, 423.
Sharan, S.K., et al. 1997. Nature 386, 804.
Ludwig, T., et al. 1997. Genes Dev. 11, 1226.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
Suzuki, A., et al. 1997. Genes Dev. 11, 1242.
Wong, A.K.C., et al. 1997. J. Biol. Chem. 272, 31941.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin. Oncol. 23, 1.
Goggins, M., et al. 1996. Cancer Res. 56, 5360.
Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
Spillman, M.A. and A.M. Bowcock. 1996. Oncogene 13, 1639.
Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
Wooster, R., et al. 1995. Nature 378, 789.
Product Information
DeclarationSold under license of U.S. Patents 5,837,492 and 6,124,104.
FormLiquid
FormulationIn PBS.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (2 µg/ml)
Immunoprecipitation (2 µg)
Application CommentsThe CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST
ImmunogenHuman
Clone2B
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
OP95-100UG 04055977209709